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Science 2 December 1994:
Vol. 266. no. 5190, pp. 1569 - 1573
DOI: 10.1126/science.7985028
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Articles

Science, Vol 266, Issue 5190, 1569-1573
Copyright © 1994 by American Association for the Advancement of Science

articles

Reconstitution of an operational MHC class II compartment in nonantigen-presenting cells

L Karlsson, A Peleraux, R Lindstedt, M Liljedahl, and PA Peterson

R. W. Johnson Pharmaceutical Research Institute, Scripps Research Institute, La Jolla, CA 92037.

Professional antigen-presenting cells (APCs) have a distinct compartment in which class II molecules are proposed to acquire antigenic peptides. Genetic evidence suggests that human leukocyte antigen (HLA)-DM, an unusual class II molecule, participates in this process. Peptide acquisition was reconstituted in nonprofessional APCs by transfection of class II, invariant chain (li), and H-2M, the murine equivalent of DM. The H-2M heterodimer appeared in an endosomal compartment, not at the cell surface, and the localization was independent of li. The data presented show that H-2M, class II, and li are the minimally required components for efficient formation of stable class II-peptide complexes, and thus for a functional class II compartment.


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