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Science 11 November 1994:
Vol. 266. no. 5187, pp. 1035 - 1039
DOI: 10.1126/science.7973656
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Articles

Science, Vol 266, Issue 5187, 1035-1039
Copyright © 1994 by American Association for the Advancement of Science

articles

Identification of two GTP-binding proteins in the chloroplast protein import machinery

F Kessler, G Blobel, HA Patel, and DJ Schnell

Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021.

Two of four proteins that associated with translocation intermediates during protein import across the outer chloroplast envelope membrane were identified as guanosine triphosphate (GTP)-binding proteins. Both proteins are integral membrane proteins of the outer chloroplast membrane, and both are partially exposed on the chloroplast surface where they were accessible to thermolysin digestion. Engagement of the outer membrane's import machinery by an import substrate was inhibited by slowly hydrolyzable or non-hydrolyzable GTP analogs. Thus, these GTP-binding proteins may function in protein import into chloroplasts.


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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
The Early Stage of Chloroplast Protein Import Involves Com70.
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J. Biol. Chem. 272, 2808-2813
   Abstract »    Full Text »    PDF »
In Vitro Interaction between a Chloroplast Transit Peptide and Chloroplast Outer Envelope Lipids Is Sequence-specific and Lipid Class-dependent.
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   Abstract »    Full Text »    PDF »
Protein Translocation at the Envelope and Thylakoid Membranes of Chloroplasts.
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