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Science 11 November 1994:
Vol. 266. no. 5187, pp. 1007 - 1012
DOI: 10.1126/science.7973649
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Articles

Science, Vol 266, Issue 5187, 1007-1012
Copyright © 1994 by American Association for the Advancement of Science

articles

Isolation of components of the chloroplast protein import machinery

DJ Schnell, F Kessler, and G Blobel

Department of Biological Sciences, Rutgers, State University of New Jersey, Newark 07102.

Components of the protein import machinery of the chloroplast were isolated by a procedure in which the import machinery was engaged in vitro with a tagged import substrate under conditions that yielded largely chloroplast envelope-bound import intermediates. Subsequent detergent solubilization of envelope membranes showed that six envelope polypeptides copurified specifically and, apparently, stoichiometrically with the import intermediates. Four of these polypeptides are components of the outer membrane import machinery and are associated with early import intermediates. Two of these polypeptides have been characterized. One is a homolog of the heat shock protein hsp70; the other one is a channel-protein candidate.


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J. Cell Biol. 154, 309-316
   Abstract »    Full Text »    PDF »
Arabidopsis Genes Encoding Components of the Chloroplastic Protein Import Apparatus.
D. Jackson-Constan and K. Keegstra (2001)
Plant Physiology 125, 1567-1576
   Abstract »    Full Text »
Maize Non-Photosynthetic Ferredoxin Precursor Is Mis-Sorted to the Intermembrane Space of Chloroplasts in the Presence of Light.
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   Abstract »    Full Text »
Chloroplast precursor proteins compete to form early import intermediates in isolated pea chloroplasts.
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J. Exp. Bot. 52, 47-56
   Abstract »    Full Text »    PDF »
The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates.
P. E. Row and J. C. Gray (2001)
J. Exp. Bot. 52, 57-66
   Abstract »    Full Text »    PDF »
Insertion of OEP14 into the Outer Envelope Membrane Is Mediated by Proteinaceous Components of Chloroplasts.
S.-L. Tu and H.-m. Li (2000)
PLANT CELL 12, 1951-1960
   Abstract »    Full Text »
Identification of a Hsp70 Recognition Domain within the Rubisco Small Subunit Transit Peptide.
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Plant Physiology 122, 1289-1300
   Abstract »    Full Text »
The Transit Sequence of Ferredoxin Contains Different Domains for Translocation across the Outer and Inner Membrane of the Chloroplast Envelope.
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J. Biol. Chem. 275, 10265-10271
   Abstract »    Full Text »    PDF »
Toc64, a New Component of the Protein Translocon of Chloroplasts.
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J. Cell Biol. 148, 1213-1222
   Abstract »    Full Text »    PDF »
Initial Binding of Preproteins Involving the Toc159 Receptor Can Be Bypassed during Protein Import into Chloroplasts.
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Plant Physiology 122, 813-822
   Abstract »    Full Text »    PDF »
Tic40, a New "Old" Subunit of the Chloroplast Protein Import Translocon.
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J. Biol. Chem. 274, 37467-37472
   Abstract »    Full Text »    PDF »
The C Terminus of a Chloroplast Precursor Modulates Its Interaction with the Translocation Apparatus and PIRAC.
C. Dabney-Smith, P. W. J. van den Wijngaard, Y. Treece, W. J. Vredenberg, and B. D. Bruce (1999)
J. Biol. Chem. 274, 32351-32359
   Abstract »    Full Text »    PDF »
Stromal Processing Peptidase Binds Transit Peptides and Initiates Their Atp-Dependent Turnover in Chloroplasts.
S. Richter and G. K. Lamppa (1999)
J. Cell Biol. 147, 33-44
   Abstract »    Full Text »    PDF »
GTP Promotes the Formation of Early-Import Intermediates But Is Not Required during the Translocation Step of Protein Import into Chloroplasts.
M. E. Young, K. Keegstra, and J. E. Froehlich (1999)
Plant Physiology 121, 237-244
   Abstract »    Full Text »
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L.-Y. Tsai, S.-L. Tu, and H.-m. Li (1999)
J. Biol. Chem. 274, 18735-18740
   Abstract »    Full Text »    PDF »
A Chloroplast-Targeted Heat Shock Protein 70 (HSP70) Contributes to the Photoprotection and Repair of Photosystem II during and after Photoinhibition.
M. Schroda, O. Vallon, F.-A. Wollman, and C. F. Beck (1999)
PLANT CELL 11, 1165-1178
   Abstract »    Full Text »
Protein Import and Routing Systems of Chloroplasts.
K. Keegstra and K. Cline (1999)
PLANT CELL 11, 557-570
   Full Text »
The evolutionary origin of the protein-translocating channel of chloroplastic envelope membranes: Identification of a cyanobacterial homolog.
S. Reumann, J. Davila-Aponte, and K. Keegstra (1999)
PNAS 96, 784-789
   Abstract »    Full Text »    PDF »
Origin of a chloroplast protein importer.
B. Bolter, J. Soll, A. Schulz, S. Hinnah, and R. Wagner (1998)
PNAS 95, 15831-15836
   Abstract »    Full Text »    PDF »
Tic20 and Tic22 Are New Components of the Protein Import Apparatus at the Chloroplast Inner Envelope Membrane.
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J. Cell Biol. 143, 991-1002
   Abstract »    Full Text »    PDF »
An Arabidopsis Mutant Defective in the Plastid General Protein Import Apparatus.
P. Jarvis, L. Chen, H. Li, C. A. Peto, C. Fankhauser, and J. Chory (1998)
Science 282, 100-103
   Abstract »    Full Text »
Domains of a Transit Sequence Required for in Vivo Import in Arabidopsis Chloroplasts.
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Plant Physiology 118, 691-699
   Abstract »    Full Text »
The Hydrophilic Domain of Tic110, an Inner Envelope Membrane Component of the Chloroplastic Protein Translocation Apparatus, Faces the Stromal Compartment.
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   Abstract »    Full Text »    PDF »
The Organization and Evolution of the Spinach Stress 70 Molecular Chaperone Gene Family.
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Analysis of the Interactions of Preproteins with the Import Machinery over the Course of Protein Import into Chloroplasts.
A. Kouranov and D. J. Schnell (1997)
J. Cell Biol. 139, 1677-1685
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P. W. J. van den Wijngaard and W. J. Vredenberg (1997)
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   Abstract »    Full Text »    PDF »
Isolation and characterization of an amino acid-selective channel protein present in the chloroplastic outer envelope membrane.
K. Pohlmeyer, J. Soll, T. Steinkamp, S. Hinnah, and R. Wagner (1997)
PNAS 94, 9504-9509
   Abstract »    Full Text »    PDF »
Alterations in the Chlamydomonas Plastocyanin Transit Peptide Have Distinct Effects on in Vitro Import and in Vivo Protein Accumulation.
S. D. Lawrence and K. L. Kindle (1997)
J. Biol. Chem. 272, 20357-20363
   Abstract »    Full Text »    PDF »
In vivo zippering of inner and outer mitochondrial membranes by a stable translocation intermediate.
N. Schulke, N. B. V. Sepuri, and D. Pain (1997)
PNAS 94, 7314-7319
   Abstract »    Full Text »    PDF »
A Novel Chloroplastic Outer Membrane-targeting Signal That Functions at Both Termini of Passenger Polypeptides.
H.-m. Li and L.-J. Chen (1997)
J. Biol. Chem. 272, 10968-10974
   Abstract »    Full Text »    PDF »
Identification of a Translocation Intermediate Occupying Functional Protein Import Sites in the Chloroplastic Envelope Membrane.
J. E. Froehlich and K. Keegstra (1997)
J. Biol. Chem. 272, 8077-8082
   Abstract »    Full Text »    PDF »
Identification of Protein Transport Complexes in the Chloroplastic Envelope Membranes via Chemical Cross-Linking.
M. Akita, E. Nielsen, and K. Keegstra (1997)
J. Cell Biol. 136, 983-994
   Abstract »    Full Text »    PDF »
Insertion of the 34-kDa Chloroplast Protein Import Component, IAP34, into the Chloroplast Outer Membrane Is Dependent on Its Intrinsic GTP-binding Capacity.
D. Chen and D. J. Schnell (1997)
J. Biol. Chem. 272, 6614-6620
   Abstract »    Full Text »    PDF »
The Early Stage of Chloroplast Protein Import Involves Com70.
L. Kourtz and K. Ko (1997)
J. Biol. Chem. 272, 2808-2813
   Abstract »    Full Text »    PDF »
In Vitro Interaction between a Chloroplast Transit Peptide and Chloroplast Outer Envelope Lipids Is Sequence-specific and Lipid Class-dependent.
P. Pinnaduwage and B. D. Bruce (1996)
J. Biol. Chem. 271, 32907-32915
   Abstract »    Full Text »    PDF »
Protein Translocation at the Envelope and Thylakoid Membranes of Chloroplasts.
A. Kouranov and D. J. Schnell (1996)
J. Biol. Chem. 271, 31009-31012
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A Protein Import Receptor of Chloroplasts Is Inserted into the Outer Envelope Membrane by a Novel Pathway.
E. Muckel and J. Soll (1996)
J. Biol. Chem. 271, 23846-23852
   Abstract »    Full Text »    PDF »
Phosphorylation of the Transit Sequence of Chloroplast Precursor Proteins.
K. Waegemann and Jür. Soll (1996)
J. Biol. Chem. 271, 6545-6554
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J. Biol. Chem. 270, 28601-28608
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Characterization of the Import Process of a Transit Peptide into Chloroplasts.
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J. Biol. Chem. 270, 22368-22373
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A Voltage-dependent Porin-like Channel in the Inner Envelope Membrane of Plant Chloroplasts.
B. Fuks and F. Homblé (1995)
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Unidirectional and Bidirectional Protein Traffic across Membranes.
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