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Science 4 November 1994:
Vol. 266. no. 5186, pp. 796 - 798
DOI: 10.1126/science.7973633
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Articles

Science, Vol 266, Issue 5186, 796-798
Copyright © 1994 by American Association for the Advancement of Science

articles

SecA homolog in protein transport within chloroplasts: evidence for endosymbiont-derived sorting

J Yuan, R Henry, M McCaffery, and K Cline

Plant Molecular and Cellular Biology Program, University of Florida, Gainesville 32611.

The SecA protein is an essential, azide-sensitive component of the bacterial protein translocation machinery. A SecA protein homolog (CPSecA) now identified in pea chloroplasts was purified to homogeneity. CPSecA supported protein transport into thylakoids, the chloroplast internal membrane network, in an azide-sensitive fashion. Only one of three pathways for protein transport into thylakoids uses the CPSecA mechanism. The use of a bacteria-homologous mechanism in intrachloroplast protein transport provides evidence for conservative sorting of proteins within chloroplasts.


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Component Specificity for the Thylakoidal Sec and Delta Ph-Dependent Protein Transport Pathways.
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The Sec-independent Twin-arginine Translocation System Can Transport Both Tightly Folded and Malfolded Proteins across the Thylakoid Membrane.
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An Essential Component of a Novel Bacterial Protein Export System with Homologues in Plastids and Mitochondria.
E. G. Bogsch, F. Sargent, N. R. Stanley, B. C. Berks, C. Robinson, and T. Palmer (1998)
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A SecY Homologue Is Required for the Elaboration of the Chloroplast Thylakoid Membrane and for Normal Chloroplast Gene Expression.
L. M. Roy and A. Barkan (1998)
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Identification of a Role for an Azide-Sensitive Factor in the Thylakoid Transport of the 17-Kilodalton Subunit of the Photosynthetic Oxygen-Evolving Complex.
E. A. Leheny, S. A. Teter, and S. M. Theg (1998)
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Ccs1, a Nuclear Gene Required for the Post-translational Assembly of Chloroplast c-Type Cytochromes.
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Chloroplast SRP54 Interacts with a Specific Subset of Thylakoid Precursor Proteins.
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Protein Translocation at the Envelope and Thylakoid Membranes of Chloroplasts.
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J. Biol. Chem. 271, 31009-31012
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Molecular Genetic Analysis of Plastocyanin Biosynthesis in Chlamydomonas reinhardtii.
H. H. Li, J. Quinn, D. Culler, J. Girard-Bascou, and S. Merchant (1996)
J. Biol. Chem. 271, 31283-31289
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Hydrophobic Core but Not Amino-terminal Charged Residues Are Required for Translocation of an Integral Thylakoid Membrane Protein in Vivo.
B. Baillet and B. D. Kohorn (1996)
J. Biol. Chem. 271, 18375-18378
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Sec-dependent Thylakoid Protein Translocation.
A. Mant, I. Schmidt, R. G. Herrmann, C. Robinson, and R. B. Klösgen (1995)
J. Biol. Chem. 270, 23275-23281
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Isolation and Characterization of a cDNA Encoding the SecA Protein from Spinach Chloroplasts.
J. Berghfer, I. Karnauchov, R. G. Herrmann, and R. B. Klsgen (1995)
J. Biol. Chem. 270, 18341-18346
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A SecY Homolog in Arabidopsis thaliana.
V. Laidler, A. M. Chaddock, T. G. Knott, D. Walker, and C. Robinson (1995)
J. Biol. Chem. 270, 17664-17667
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In Vitro Reconstitution of Insertion and Processing of Cytochrome f in a Homologous Chloroplast Translation System.
T. Rohl and K. J. van Wijk (2001)
J. Biol. Chem. 276, 35465-35472
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