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Science 4 November 1994:
Vol. 266. no. 5186, pp. 771 - 773
DOI: 10.1126/science.7973628
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Articles

Science, Vol 266, Issue 5186, 771-773
Copyright © 1994 by American Association for the Advancement of Science

articles

Direct measurement of the forces between complementary strands of DNA

GU Lee, LA Chrisey, and RJ Colton

Chemistry Division, Naval Research Laboratory, Washington, DC 20375.

Interaction forces between single strands of DNA were measured with the atomic force microscope by a procedure in which DNA oligonucleotides were covalently attached to a spherical probe and surface. Adhesive forces measured between complementary 20-base strands fell into three distinct distributions centered at 1.52, 1.11, and 0.83 nano-newtons, which are associated with the rupture of the interchain interaction between a single pair of molecules involving 20, 16, and 12 base pairs, respectively. When a third long DNA molecule was coupled between complementary surfaces, both intra- and interchain forces were observed. The intrachain interaction resulting from the molecule's elasticity manifested itself as a long-range cohesive force.


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