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Science 28 October 1994:
Vol. 266. no. 5185, pp. 650 - 653
DOI: 10.1126/science.7939720
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Articles

Science, Vol 266, Issue 5185, 650-653
Copyright © 1994 by American Association for the Advancement of Science

articles

Transgenic X. laevis embryos from eggs transplanted with nuclei of transfected cultured cells

KL Kroll and JC Gerhart

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Transgenic Xenopus laevis embryos were produced by transplantation of transfected cultured cell nuclei into unfertilized eggs. A Xenopus cell line, X-C, was stably transfected with plasmids containing a hygromycin-resistance gene and genes for either beta-galactosidase with a heat shock promoter or chloramphenicol acetyltransferase (CAT) with a muscle-specific actin promoter. Nuclei transplanted from these cells into unfertilized eggs directed development of embryos containing stably integrated copies of the plasmids in each cell. Transgenic embryos showed somite-specific expression of CAT and uniform expression of beta-galactosidase. Transgenic embryos produced by nuclear transplantation should be useful for testing the function of cloned genes in amphibian development.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Fertile and diploid nuclear transplants derived from embryonic cells of a small laboratory fish, medaka (Oryziaslatipes).
Y. Wakamatsu, B. Ju, I. Pristyaznhyuk, K. Niwa, T. Ladygina, M. Kinoshita, K. Araki, and K. Ozato (2001)
PNAS 98, 1071-1076
   Abstract »    Full Text »    PDF »
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
K. Kroll and E Amaya (1996)
Development 122, 3173-3183
   Abstract »    PDF »
Blastomere derivation and domains of gene expression in the Spemann Organizer of Xenopus laevis.
M. Vodicka and J. Gerhart (1995)
Development 121, 3505-3518
   Abstract »    PDF »


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