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Science 7 October 1994:
Vol. 266. no. 5182, pp. 117 - 119
DOI: 10.1126/science.7939629
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Articles

Science, Vol 266, Issue 5182, 117-119
Copyright © 1994 by American Association for the Advancement of Science

articles

Identification of the ron gene product as the receptor for the human macrophage stimulating protein

MH Wang, C Ronsin, MC Gesnel, L Coupey, A Skeel, EJ Leonard, and R Breathnach

Immunopathology Section, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702.

Macrophage-stimulating protein (MSP) is a member of the hepatocyte growth factor-scatter factor (HGF-SF) family. Labeled MSP bound to Madin-Darby canine kidney (MDCK) cells transfected with complementary DNA encoding Ron, a cell membrane protein tyrosine kinase. Cross-linking of 125I-labeled MSP to transfected cells (MDCK-RE7 cells) and immunoprecipitation by antibodies to Ron revealed a 220-kilodalton complex, a size consistent with that of MSP (80 kilodaltons) cross-linked to the beta chain of Ron (150 kilodaltons). The binding of 125I-labeled MSP to MDCK-RE7 cells was inhibited by unlabeled MSP, but not by HGF-SF. MSP caused phosphorylation of the beta chain of Ron and induced migration of MDCK-RE7 cells. These results establish the ron gene product as a specific cell-surface receptor for MSP.


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