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Science 7 October 1994:
Vol. 266. no. 5182, pp. 114 - 117
DOI: 10.1126/science.7524149
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Articles

Science, Vol 266, Issue 5182, 114-117
Copyright © 1994 by American Association for the Advancement of Science

articles

RNA editing: transfer of genetic information from gRNA to precursor mRNA in vitro

SD Seiwert and K Stuart

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06536-0182.

RNA editing in the mitochondrion of Trypanosoma brucei extensively alters the adenosine triphosphate synthase (ATPase) subunit 6 precursor messenger RNA (pre-mRNA) by addition of 447 uridines and removal of 28 uridines. In vivo, the guide RNA gA6[14] is thought to specify the deletion of two uridines from the editing site closest to the 3' end. In this study, an in vitro system was developed that accurately removed uridines from this editing site in synthetic ATPase 6 pre-mRNA when gA6[14] and ATP were added. Mutations in both the guide RNA and the pre-mRNA editing site suggest that base-pairing interactions control the number of uridines deleted in vitro. Thus, guide RNAs are required for RNA editing and for the transfer of genetic information to pre-mRNAs.


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S. S. Leung and D. J. Koslowsky (2001)
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RNA-binding properties of the mitochondrial Y-box protein RBP16.
M. Pelletier, M. M. Miller, and L. K. Read (2000)
Nucleic Acids Res. 28, 1266-1275
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The Mitochondrial RNA Ligase from Leishmania tarentolae Can Join RNA Molecules Bridged by a Complementary RNA.
V. Blanc, J. D. Alfonzo, R. Aphasizhev, and L. Simpson (1999)
J. Biol. Chem. 274, 24289-24296
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Trypanosoma brucei RBP16 Is a Mitochondrial Y-box Family Protein with Guide RNA Binding Activity.
M. L. Hayman and L. K. Read (1999)
J. Biol. Chem. 274, 12067-12074
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Association of Guide RNA Binding Protein gBP21 with Active RNA Editing Complexes in Trypanosoma brucei.
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Trypanosoma brucei gBP21. AN ARGININE-RICH MITOCHONDRIAL PROTEIN THAT BINDS TO GUIDE RNA WITH HIGH AFFINITY.
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Trypanosoma brucei RNA Editing.
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Isolation and Characterization of a U-specific 3'-5'-Exonuclease from Mitochondria of Leishmania tarentolae.
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