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Science 30 September 1994:
Vol. 265. no. 5181, pp. 2096 - 2098
DOI: 10.1126/science.7522347
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Articles

Science, Vol 265, Issue 5181, 2096-2098
Copyright © 1994 by American Association for the Advancement of Science

articles

Alignment and sensitive detection of DNA by a moving interface

A Bensimon, A Simon, A Chiffaudel, V Croquette, F Heslot, and D Bensimon

Institut Pasteur, Paris, France.

In a process called "molecular combining," DNA molecules attached at one end to a solid surface were extended and aligned by a receding air-water interface and left to dry on the surface. Molecular combing was observed to extend the length of the bacteriophage lambda DNA molecule to 21.5 +/- 0.5 micrometers (unextended length, 16.2 micrometers). With the combing process, it was possible to (i) extend a chromosomal Escherichia coli DNA fragment (10(6) base pairs) and (ii) detect a minute quantity of DNA (10(3) molecules). These results open the way for a faster physical mapping of the genome and for the detection of small quantities of target DNA from a population of molecules.


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