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Science 30 September 1994:
Vol. 265. no. 5181, pp. 2082 - 2085
DOI: 10.1126/science.8091230
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Articles

Science, Vol 265, Issue 5181, 2082-2085
Copyright © 1994 by American Association for the Advancement of Science

articles

Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease

AJ Bardwell, L Bardwell, AE Tomkinson, and EC Friedberg

Laboratory of Molecular Pathology, University of Texas Southwestern Medical Center at Dallas 75235.

The RAD1 and RAD10 genes of Saccharomyces cerevisiae are required for both nucleotide excision repair and certain mitotic recombination events. Here, model recombination and repair intermediates were used to show that Rad1-Rad10-mediated cleavage occurs at duplex-single-strand junctions. Moreover, cleavage occurs only on the strand containing the 3' single-stranded tail. Thus, both biochemical and genetic evidence indicate a role for the Rad1-Rad10 complex in the cleavage of specific recombination intermediates. Furthermore, these data suggest that Rad1-Rad10 endonuclease incises DNA 5' to damaged bases during nucleotide excision repair.


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A novel mechanism for telomere size control in Saccharomyces cerevisiae..
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Replication Protein A Confers Structure-specific Endonuclease Activities to the XPF-ERCC1 and XPG Subunits of Human DNA Repair Excision Nuclease.
T. Matsunaga, C.-H. Park, T. Bessho, D. Mu, and A. Sancar (1996)
J. Biol. Chem. 271, 11047-11050
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Reconstitution of TFIIH and Requirement of Its DNA Helicase Subunits, Rad3 and Rad25, in the Incision Step of Nucleotide Excision Repair.
P. Sung, S. N. Guzder, L. Prakash, and S. Prakash (1996)
J. Biol. Chem. 271, 10821-10826
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Analysis of Incision Sites Produced by Human Cell Extracts and Purified Proteins during Nucleotide Excision Repair of a 1,3-Intrastrand d(GpTpG)-Cisplatin Adduct.
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Structure-specific Nuclease Activity in Yeast Nucleotide Excision Repair Protein Rad2.
Y. Habraken, P. Sung, L. Prakash, and S. Prakash (1995)
J. Biol. Chem. 270, 30194-30198
   Abstract »    Full Text »    PDF »
Role of the Rad1 and Rad10 Proteins in Nucleotide Excision Repair and Recombination.
A. A. Davies, E. C. Friedberg, A. E. Tomkinson, R. D. Wood, and S. C. West (1995)
J. Biol. Chem. 270, 24638-24641
   Abstract »    Full Text »    PDF »
Purification and Characterization of the XPF-ERCC1 Complex of Human DNA Repair Excision Nuclease.
C.-H. Park, T. Bessho, T. Matsunaga, and A. Sancar (1995)
J. Biol. Chem. 270, 22657-22660
   Abstract »    Full Text »    PDF »
Human DNA Repair Excision Nuclease.
T. Matsunaga, D. Mu, C.-H. Park, J. T. Reardon, and A. Sancar (1995)
J. Biol. Chem. 270, 20862-20869
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DNA Structural Elements Required for FEN-1 Binding.
J. J. Harrington and M. R. Lieber (1995)
J. Biol. Chem. 270, 4503-4508
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The General Transcription-Repair Factor TFIIH Is Recruited to the Excision Repair Complex by the XPA Protein Independent of the TFIIE Transcription Factor.
C.-H. Park, D. Mu, J. T. Reardon, and A. Sancar (1995)
J. Biol. Chem. 270, 4896-4902
   Abstract »    Full Text »    PDF »
Mechanisms of DNA excision repair.
A Sancar (1994)
Science 266, 1954-1956
   PDF »
Repair of an Interstrand DNA Cross-link Initiated by ERCC1-XPF Repair/Recombination Nuclease.
I. Kuraoka, W. R. Kobertz, R. R. Ariza, M. Biggerstaff, J. M. Essigmann, and R. D. Wood (2000)
J. Biol. Chem. 275, 26632-26636
   Abstract »    Full Text »    PDF »
Transcription-induced Cleavage of Immunoglobulin Switch Regions by Nucleotide Excision Repair Nucleases in Vitro.
M. Tian and F. W. Alt (2000)
J. Biol. Chem. 275, 24163-24172
   Abstract »    Full Text »    PDF »


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