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Science 23 September 1994:
Vol. 265. no. 5180, pp. 1863 - 1866
DOI: 10.1126/science.8091212
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Articles

Science, Vol 265, Issue 5180, 1863-1866
Copyright © 1994 by American Association for the Advancement of Science

articles

Synergistic activation of transcription by bacteriophage lambda cI protein and E. coli cAMP receptor protein

JK Joung, DM Koepp, and A Hochschild

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

Two heterologous prokaryotic activators, the bacteriophage lambda cI protein (lambda cI) and the Escherichia coli cyclic AMP receptor protein (CRP), were shown to activate transcription synergistically from an artificial promoter bearing binding sites for both proteins. The synergy depends on a functional activation (positive control) surface on each activator. These results imply that both proteins interact directly with RNA polymerase and thus suggest a precise mechanism for transcriptional synergy: the interaction of two activators with two distinct surfaces of RNA polymerase.


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