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Science 16 September 1994:
Vol. 265. no. 5179, pp. 1709 - 1712
DOI: 10.1126/science.8085157
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Articles

Science, Vol 265, Issue 5179, 1709-1712
Copyright © 1994 by American Association for the Advancement of Science

articles

Coaxially stacked RNA helices in the catalytic center of the Tetrahymena ribozyme

FL Murphy, YH Wang, JD Griffith, and TR Cech

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.

Coaxial stacking of helical elements is a determinant of three-dimensional structure in RNA. In the catalytic center of the Tetrahymena group I intron, helices P4 and P6 are part of a tertiary structural domain that folds independently of the remainder of the intron. When P4 and P6 were fused with a phosphodiester linkage, the resulting RNA retained the detailed tertiary interactions characteristic of the native P4-P6 domain and even required lower magnesium ion concentrations for folding. These results indicate that P4 and P6 are coaxial in the P4-P6 domain and, therefore, in the native ribozyme. Helix fusion could provide a general method for identifying pairs of coaxially stacked helices in biological RNA molecules.


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