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Science 19 August 1994:
Vol. 265. no. 5175, pp. 1091 - 1093
DOI: 10.1126/science.8066446
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Articles

Science, Vol 265, Issue 5175, 1091-1093
Copyright © 1994 by American Association for the Advancement of Science

articles

MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast

TA Prolla, Q Pang, E Alani, RD Kolodner, and RM Liskay

Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06511.

The discovery that mutations in DNA mismatch repair genes can cause hereditary nonpolyposis colorectal cancer has stimulated interest in understanding the mechanism of DNA mismatch repair in eukaryotes. In the yeast Saccharomyces cerevisiae, DNA mismatch repair requires the MSH2, MLH1, and PMS1 proteins. Experiments revealed that the yeast MLH1 and PMS1 proteins physically associate, possibly forming a heterodimer, and that MLH1 and PMS1 act in concert to bind a MSH2-heteroduplex complex containing a G-T mismatch. Thus, MSH2, MLH1, and PMS1 are likely to form a ternary complex during the initiation of eukaryotic DNA mismatch repair.


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Physical interaction between components of DNA mismatch repair and nucleotide excision repair.
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PNAS 95, 14278-14283
   Abstract »    Full Text »    PDF »
The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations.
H. Flores-Rozas and R. D. Kolodner (1998)
PNAS 95, 12404-12409
   Abstract »    Full Text »    PDF »
MutS and MutL Activate DNA Helicase II in a Mismatch-dependent Manner.
M. Yamaguchi, V. Dao, and P. Modrich (1998)
J. Biol. Chem. 273, 9197-9201
   Abstract »    Full Text »    PDF »
The Chromosome Bias of Misincorporations During Double-Strand Break Repair Is Not Altered in Mismatch Repair–Defective Strains of Saccharomyces cerevisiae.
C. B. McGill, S. L. Holbeck, and J. N. Strathern (1998)
Genetics 148, 1525-1533
   Abstract »    Full Text »    PDF »
Meiotic chromosomes: it takes two to tango.
G. S. Roeder (1997)
Genes & Dev. 11, 2600-2621
   Full Text »    PDF »
Strand-specific Mismatch Repair in Mammalian Cells.
P. Modrich (1997)
J. Biol. Chem. 272, 24727-24730
   Full Text »    PDF »
A Conditionally Expressed Third Partner Stabilizes or Prevents the Formation of a Transcriptional Activator in a Three-hybrid System.
F. Tirode, C. Malaguti, F. Romero, R. Attar, J. Camonis, and J. M. Egly (1997)
J. Biol. Chem. 272, 22995-22999
   Abstract »    Full Text »    PDF »
Mlh1 is unique among mismatch repair proteins in its ability to promote crossing-over during meiosis..
N Hunter and R H Borts (1997)
Genes & Dev. 11, 1573-1582
   Abstract »    PDF »
Elevated levels of mutation in multiple tissues of mice deficient in the DNA mismatch repair gene Pms2.
L. Narayanan, J. A. Fritzell, S. M. Baker, R. M. Liskay, and P. M. Glazer (1997)
PNAS 94, 3122-3127
   Abstract »    Full Text »    PDF »
Biochemistry and genetics of eukaryotic mismatch repair..
R Kolodner (1996)
Genes & Dev. 10, 1433-1442
   PDF »
Requirement of the Yeast MSH3 and MSH6 Genes for MSH2-dependent Genomic Stability.
R. E. Johnson, G. K. Kovvali, L. Prakash, and S. Prakash (1996)
J. Biol. Chem. 271, 7285-7288
   Abstract »    Full Text »    PDF »
Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair..
G T Marsischky, N Filosi, M F Kane, and R Kolodner (1996)
Genes & Dev. 10, 407-420
   Abstract »    PDF »
hMSH2-independent DNA Mismatch Recognition by Human Proteins.
N. E. O'Regan, P. Branch, P. Macpherson, and P. Karran (1996)
J. Biol. Chem. 271, 1789-1796
   Abstract »    Full Text »    PDF »
A hPMS2 Mutant Cell Line Is Defective in Strand-specific Mismatch Repair.
J. I. Risinger, A. Umar, J. C. Barrett, and T. A. Kunkel (1995)
J. Biol. Chem. 270, 18183-18186
   Abstract »    Full Text »    PDF »
GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells.
F Palombo, P Gallinari, I Iaccarino, T Lettieri, M Hughes, A D'Arrigo, O Truong, J. Hsuan, and J Jiricny (1995)
Science 268, 1912-1914
   Abstract »    PDF »
Mismatch repair deficiency in phenotypically normal human cells.
R Parsons, G. Li, M Longley, P Modrich, B Liu, T Berk, Hamilton SR, K. Kinzler, and B Vogelstein (1995)
Science 268, 738-740
   Abstract »    PDF »
Construction of a Human Genomic Library of Clones Containing Poly(dG-dA)[IMAGE]Poly(dT-dC) Tracts by Mg[IMAGE]-dependent Triplex Affinity Capture.
N. Nishikawa, M. Oishi, and R. Kiyama (1995)
J. Biol. Chem. 270, 9258-9264
   Abstract »    Full Text »    PDF »
The Saccharomyces cerevisiae Msh2 protein specifically binds to duplex oligonucleotides containing mismatched DNA base pairs and insertions..
E Alani, N W Chi, and R Kolodner (1995)
Genes & Dev. 9, 234-247
   Abstract »    PDF »
Human Mismatch Repair Genes and Their Association with Hereditary Non-Polyposis Colon Cancer.
R.D. Kolodner, N.R. Hall, J. Lipford, M.F. Kane, M.R.S. Rao, P. Morrison, L. Wirth, P.J. Finan, J. Burn, P. Chapman, et al. (1994)
Cold Spring Harb Symp Quant Biol 59, 331-338
   Abstract »    PDF »
Identification of Mismatch Repair Protein Complexes in HeLa Nuclear Extracts and Their Interaction with Heteroduplex DNA.
N. Matton, J. Simonetti, and K. Williams (2000)
J. Biol. Chem. 275, 17808-17813
   Abstract »    Full Text »    PDF »
Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiae.
P. T. Tran, J. A. Simon, and R. M. Liskay (2001)
PNAS 98, 9760-9765
   Abstract »    Full Text »    PDF »
Meiotic recombination frequencies are affected by nutritional states in Saccharomycescerevisiae.
M. F. F. Abdullah and R. H. Borts (2001)
PNAS 98, 14524-14529
   Abstract »    Full Text »    PDF »


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