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Science 5 August 1994:
Vol. 265. no. 5173, pp. 796 - 799
DOI: 10.1126/science.8047885
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Articles

Science, Vol 265, Issue 5173, 796-799
Copyright © 1994 by American Association for the Advancement of Science

articles

Stimulation of RNA polymerase II elongation by chromosomal protein HMG-14

HF Ding, S Rimsky, SC Batson, M Bustin, and U Hansen

Division of Molecular Genetics, Dana-Farber Cancer Institute, Boston, MA.

The high-mobility group protein 14 (HMG-14) is a non-histone chromosomal protein that is preferentially associated with transcriptionally active chromatin. To assess the effect of HMG-14 on transcription by RNA polymerase II, in vivo-assembled chromatin with elevated amounts of HMG-14 was obtained. Here it is shown that HMG-14 enhanced transcription on chromatin templates but not on DNA templates. This protein stimulated the rate of elongation by RNA polymerase II but not the level of initiation of transcription. These findings suggest that the association of HMG-14 with nucleosomes is part of the cellular process involved in the generation of transcriptionally active chromatin.


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