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Science 5 August 1994:
Vol. 265. no. 5173, pp. 777 - 780
DOI: 10.1126/science.7519361
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Articles

Science, Vol 265, Issue 5173, 777-780
Copyright © 1994 by American Association for the Advancement of Science

articles

NMR solution structure of a peptide nucleic acid complexed with RNA

SC Brown, SA Thomson, JM Veal, and DG Davis

Glaxo Research Institute, Research Triangle Park, NC 27709.

Peptide nucleic acids (PNA) incorporating nucleic acid bases into an achiral polyamide backbone bind to DNA in a sequence-dependent manner. The structure of a PNA-ribonucleic acid (RNA) complex was determined with nuclear magnetic resonance methods. A hexameric PNA formed a 1:1 complex with a complementary RNA that is an antiparallel, right-handed double helix with Watson-Crick base pairing similar to the "A" form structure of RNA duplexes. The achiral PNA backbone assumed a distinct conformation upon binding that differed from previously proposed models and provides a basis for further structure-based design of antisense agents.


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