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Science 29 July 1994:
Vol. 265. no. 5172, pp. 669 - 671
DOI: 10.1126/science.8036515
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Articles

Science, Vol 265, Issue 5172, 669-671
Copyright © 1994 by American Association for the Advancement of Science

articles

Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene

YH Wang, S Amirhaeri, S Kang, RD Wells, and JD Griffith

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599.

The expansion of CTG repeats in DNA occurs in or near genes involved in several human diseases, including myotonic dystrophy and Huntington's disease. Nucleosomes, the basic structural element of chromosomes, consist of 146 base pairs of DNA coiled about an octamer of histone proteins and mediate general transcriptional repression. Electron microscopy was used to examine in vitro the nucleosome assembly of DNA containing repeating CTG triplets. The efficiency of nucleosome formation increased with expanded triplet blocks, suggesting that such blocks may repress transcription through the creation of stable nucleosomes.


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