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Science 17 June 1994:
Vol. 264. no. 5166, pp. 1768 - 1771
DOI: 10.1126/science.8209257
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Articles

Science, Vol 264, Issue 5166, 1768-1771
Copyright © 1994 by American Association for the Advancement of Science

articles

Silencers and domains of generalized repression

S Loo and J Rine

Department of Molecular and Cell Biology, University of California at Berkeley 94720.

Gene expression can be affected by the chromosomal position of the gene. An example of this position effect is silencing of the HML and HMR mating-type loci of Saccharomyces cerevisiae. An in vitro assay revealed that silencing induced a transcription-independent general occlusion of the DNA at HMR from sequence-specific interactions of proteins with DNA. The minimum boundaries of the silenced chromatin structure were determined, as were the contributions of the E and I silencers to the size of the silenced domain. Examination of endonuclease-sensitive sites provided evidence that neither the integrity of the chromosomal duplex nor covalent linkage of the silencers to HMR was important for maintenance of the silenced structure in vitro.


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Silencers are required for inheritance of the repressed state in yeast..
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The Saccharomyces retrotransposon Ty5 integrates preferentially into regions of silent chromatin at the telomeres and mating loci..
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Binding sites for transcription factor NTF-1/Elf-1 contribute to the ventral repression of decapentaplegic..
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The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability..
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