Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 20 May 1994:
Vol. 264. no. 5162, pp. 1137 - 1141
DOI: 10.1126/science.7513905
Prev | Table of Contents | Next

Articles

Science, Vol 264, Issue 5162, 1137-1141
Copyright © 1994 by American Association for the Advancement of Science

articles

RNAs with dual specificity and dual RNAs with similar specificity

GJ Connell and M Yarus

Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.

The biological role of RNA is delimited by its possible reactions, which can be explored by selection. A comparison of selected RNAs that bind one ligand with those that bind two related ligands suggests that a single nucleotide substitution can expand binding specificity. An RNA site with dual (joint) specificity has adenine and cytosine bases whose pKa's appear shifted upward, thereby mimicking an efficient general acid-base catalyst. The joint site also contains two conserved, looped arginine-coding triplets implicated in arginine site formation. Two selected joint RNAs are identical in some regions and distinct in others. The distinct regions, like some peptides, seem to function similarly without being similar in primary structure.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Evidence for proton transfer in the rate-limiting step of a fast-cleaving Varkud satellite ribozyme.
M. D. Smith and R. A. Collins (2007)
PNAS 104, 5818-5823
   Abstract »    Full Text »    PDF »
In vitro selection of RNA aptamers against a composite small molecule-protein surface.
K. A. Plummer, J. M. Carothers, M. Yoshimura, J. W. Szostak, and G. L. Verdine (2005)
Nucleic Acids Res. 33, 5602-5610
   Abstract »    Full Text »    PDF »
A diminutive and specific RNA binding site for L-tryptophan.
I. Majerfeld and M. Yarus (2005)
Nucleic Acids Res. 33, 5482-5493
   Abstract »    Full Text »    PDF »
A More Complex Isoleucine Aptamer with a Cognate Triplet.
M. Legiewicz and M. Yarus (2005)
J. Biol. Chem. 280, 19815-19822
   Abstract »    Full Text »    PDF »
A mechanism for the association of amino acids with their codons and the origin of the genetic code.
S. D. Copley, E. Smith, and H. J. Morowitz (2005)
PNAS 102, 4442-4447
   Abstract »    Full Text »    PDF »
An intermolecular base triple as the basis of ligand specificity and affinity in the guanine- and adenine-sensing riboswitch RNAs.
J. Noeske, C. Richter, M. A. Grundl, H. R. Nasiri, H. Schwalbe, and J. Wohnert (2005)
PNAS 102, 1372-1377
   Abstract »    Full Text »    PDF »
Evolution of aptamers with a new specificity and new secondary structures from an ATP aptamer.
Z. HUANG and J. W. SZOSTAK (2003)
RNA 9, 1456-1463
   Abstract »    Full Text »    PDF »
Phylogenetic conservation of RNA secondary and tertiary structure in the trpEDCFBA operon leader transcript in Bacillus.
J. E. SCHAAK, P. BABITZKE, and P. C. BEVILACQUA (2003)
RNA 9, 1502-1515
   Abstract »    Full Text »    PDF »
Characterization of the catalytic activity of U2 and U6 snRNAs.
S. VALADKHAN and J. L. MANLEY (2003)
RNA 9, 892-904
   Abstract »    Full Text »    PDF »
Exploring the Mechanism of the Peptidyl Transfer Reaction by Chemical Footprinting.
S.A. STROBEL, G.W. MUTH, and L. CHEN (2001)
Cold Spring Harb Symp Quant Biol 66, 109-118
   Abstract »    PDF »
On Translation by RNAs Alone.
M. YARUS (2001)
Cold Spring Harb Symp Quant Biol 66, 207-216
   Abstract »    PDF »
A Single Adenosine with a Neutral pKa in the Ribosomal Peptidyl Transferase Center.
G. W. Muth, L. Ortoleva-Donnelly, and S. A. Strobel (2000)
Science 289, 947-950
   Abstract »    Full Text »
A DNA enzyme with N-glycosylase activity.
T. L. Sheppard, P. Ordoukhanian, and G. F. Joyce (2000)
PNAS 97, 7802-7807
   Abstract »    Full Text »    PDF »
General Acid-Base Catalysis in the Mechanism of a Hepatitis Delta Virus Ribozyme.
S. Nakano, D. M. Chadalavada, and P. C. Bevilacqua (2000)
Science 287, 1493-1497
   Abstract »    Full Text »
Imidazole Rescue of a Cytosine Mutation in a Self-Cleaving Ribozyme.
A. T. Perrotta, I. Shih, and M. D. Been (1999)
Science 286, 123-126
   Abstract »    Full Text »
Specific, rapid synthesis of Phe-RNA by RNA.
M. Illangasekare and M. Yarus (1999)
PNAS 96, 5470-5475
   Abstract »    Full Text »    PDF »
Nucleic acid enzymes: Playing with a fuller deck.
G. F. Joyce (1998)
PNAS 95, 5845-5847
   Full Text »    PDF »
Oligonucleotides as Research, Diagnostic, and Therapeutic Agents.
L. Gold and L. Gold (1995)
J. Biol. Chem. 270, 13581-13584
   Full Text »    PDF »
Aminoacyl-RNA synthesis catalyzed by an RNA.
M Illangasekare, G Sanchez, T Nickles, and M Yarus (1995)
Science 267, 643-647
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)