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Science 6 May 1994:
Vol. 264. no. 5160, pp. 833 - 835
DOI: 10.1126/science.8171337
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Articles

Science, Vol 264, Issue 5160, 833-835
Copyright © 1994 by American Association for the Advancement of Science

articles

Cure of xenografted human tumors by bispecific monoclonal antibodies and human T cells

C Renner, W Jung, U Sahin, R Denfeld, C Pohl, L Trumper, F Hartmann, V Diehl, R van Lier, and M Pfreundschuh

Medizinische Klinik und Poliklinik, Universitat des Saarlandes, Homburg, Germany.

Tumor immunotherapy should increase both the number of T cells that kill the tumor and the likelihood that those cells are activated at the tumor site. Bispecific monoclonal antibodies (Bi-mAbs) were designed that bound to a Hodgkin's tumor-associated antigen (CD30) on the tumor and to either CD3 or CD28 on the T cell. Immunodeficient mice were cured of established human tumors when mice were treated with both the CD3-CD30 and the CD28-CD30 Bi-mAbs and then given human peripheral blood lymphocytes that had been incubated with the CD3-CD30 Bi-mAb and cells that expressed CD30. The enrichment of human T cells within the tumor and the fact that established tumors can be cured may indicate in situ activation of both the T cell receptor and the costimulatory pathway.


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