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Science 4 March 1994:
Vol. 263. no. 5151, pp. 1278 - 1281
DOI: 10.1126/science.8122111
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Articles

Science, Vol 263, Issue 5151, 1278-1281
Copyright © 1994 by American Association for the Advancement of Science

articles

Suppression of Ras-induced transformation of NIH 3T3 cells by activated G alpha s

J Chen and R Iyengar

Department of Biochemistry, Mount Sinai School of Medicine, City University of New York, NY 10029.

Conversion of external signals into proliferative responses may be mediated by interactions between signaling pathways that control cell proliferation. Interactions between G alpha s, the alpha subunit of the heterotrimeric guanine nucleotide binding protein that stimulates adenylyl cyclase, and Ras, an important element in growth factor signaling, were studied. Expression of activated G alpha s in NIH 3T3 cells increased intracellular concentrations of adenosine 3',5'-monophosphate (cAMP) and inhibited H-Ras-stimulated DNA synthesis and mitogen-activated protein kinase activity. Activated G alpha s and 8-Br-cAMP suppressed H-Ras-induced transformation of NIH 3T3 cells. Apparently, G alpha s inhibits proliferative signals from Ras by stimulating cAMP production and activating protein kinase A.


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