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Science 18 February 1994:
Vol. 263. no. 5149, pp. 969 - 971
DOI: 10.1126/science.8310295
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Articles

Science, Vol 263, Issue 5149, 969-971
Copyright © 1994 by American Association for the Advancement of Science

articles

Reduction in viscosity of cystic fibrosis sputum in vitro by gelsolin

CA Vasconcellos, PG Allen, ME Wohl, JM Drazen, PA Janmey, and TP Stossel

Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115.

Obstruction of airways by viscous sputum causes lung damage in patients with cystic fibrosis (CF). Sputum samples from CF patients were shown to contain filamentous actin. Human plasma gelsolin, a protein that severs actin filaments, rapidly decreased the viscosity of CF sputum samples in vitro. Gc globulin and deoxyribonuclease I, proteins that sequester monomeric actin but do not sever actin filaments, were less efficient than gelsolin in diminishing sputum viscosity. These results suggest that gelsolin may have therapeutic potential as a mucolytic agent in CF patients.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)