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Science 11 February 1994:
Vol. 263. no. 5148, pp. 811 - 814
DOI: 10.1126/science.8303298
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Articles

Science, Vol 263, Issue 5148, 811-814
Copyright © 1994 by American Association for the Advancement of Science

articles

Promoter-selective transcriptional defect in cell cycle mutant ts13 rescued by hTAFII250

EH Wang and R Tjian

Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley 94720.

The TAFII250 subunit of the human transcription factor IID (TFIID) rescues the temperature-sensitive hamster cell line ts13 and overcomes a G1 arrest. Investigation of the transcriptional properties of ts13 nuclear extracts in vitro showed that activation by the site-specific regulators Sp1 and Gal4VP16 is temperature sensitive in ts13 extracts, whereas basal transcription remains unaffected. This transcriptional defect can be rescued by purified human TFIID or by expression of wild-type TAFII250 in ts13 cells. Expression from the cyclin A but not c-fos promoter is temperature sensitive in these mutant cells. Thus, the mutation in TAFII250 appears to have gene-specific effects that may lead to the ts13 cell cycle phenotype.


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