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Science 28 January 1994:
Vol. 263. no. 5146, pp. 529 - 532
DOI: 10.1126/science.7904774
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Articles

Science, Vol 263, Issue 5146, 529-532
Copyright © 1994 by American Association for the Advancement of Science

articles

Visualization of quantal synaptic transmission by dendritic calcium imaging

TH Murphy, JM Baraban, WG Wier, and LA Blatter

Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

As changes in synaptic strength are thought to be critical for learning and memory, it would be useful to monitor the activity of individual identified synapses on mammalian central neurons. Calcium imaging of cortical neurons grown in primary culture was used to visualize the activation of individual postsynaptic elements by miniature excitatory synaptic currents elicited by spontaneous quantal release. This approach revealed that the probability of spontaneous activity differed among synapses on the same dendrite. Furthermore, synapses that undergo changes in activity induced by glutamate or phorbol ester treatment were identified.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Spontaneous and Evoked Glutamate Release Activates Two Populations of NMDA Receptors with Limited Overlap.
D. Atasoy, M. Ertunc, K. L. Moulder, J. Blackwell, C. Chung, J. Su, and E. T. Kavalali (2008)
J. Neurosci. 28, 10151-10166
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Effective release rates at single rat Schaffer collateral-CA1 synapses during sustained theta-burst activity revealed by optical imaging.
G. B. Awatramani, J. D. Boyd, K. R. Delaney, and T. H. Murphy (2007)
J. Physiol. 582, 583-595
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Nonlinear [Ca2+] Signaling in Dendrites and Spines Caused by Activity-Dependent Depression of Ca2+ Extrusion.
V. Scheuss, R. Yasuda, A. Sobczyk, and K. Svoboda (2006)
J. Neurosci. 26, 8183-8194
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Regulation of Dendritic Protein Synthesis by Miniature Synaptic Events.
M. A. Sutton, N. R. Wall, G. N. Aakalu, and E. M. Schuman (2004)
Science 304, 1979-1983
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Competition between Phasic and Asynchronous Release for Recovered Synaptic Vesicles at Developing Hippocampal Autaptic Synapses.
Y. Otsu, V. Shahrezaei, B. Li, L. A. Raymond, K. R. Delaney, and T. H. Murphy (2004)
J. Neurosci. 24, 420-433
   Abstract »    Full Text »    PDF »
Miniature Transmitter Release: Accident of Nature or Careful Design?.
Y. Otsu and T. H. Murphy (2003)
Sci. STKE 2003, pe54
   Abstract »    Full Text »    PDF »
Mind-altering miniature neurotransmitter release?.
Y. Otsu and T. H. Murphy (2003)
PNAS 100, 5589-5590
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Multimodal Quantal Release at Individual Hippocampal Synapses: Evidence for No Lateral Inhibition.
A. Abenavoli, L. Forti, M. Bossi, A. Bergamaschi, A. Villa, and A. Malgaroli (2002)
J. Neurosci. 22, 6336-6346
   Abstract »    Full Text »    PDF »
AMPA Receptor-Mediated Miniature Synaptic Calcium Transients in GluR2 Null Mice.
S. Wang, Z. Jia, J. Roder, and T. H. Murphy (2002)
J Neurophysiol 88, 29-40
   Abstract »    Full Text »    PDF »
Coupling of L-type voltage-sensitive calcium channels to P2X2 purinoceptors in PC-12 cells.
E.-M. Hur, T.-J. Park, and K.-T. Kim (2001)
Am J Physiol Cell Physiol 280, C1121-C1129
   Abstract »    Full Text »    PDF »
A Calcium-Dependent Feedback Mechanism Participates in Shaping Single NMDA Miniature EPSCs.
M. Umemiya, N. Chen, L. A. Raymond, and T. H. Murphy (2001)
J. Neurosci. 21, 1-9
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Dynamics of dendritic calcium transients evoked by quantal release at excitatory hippocampal synapses.
V. N. Murthy, T. J. Sejnowski, and C. F. Stevens (2000)
PNAS 97, 901-906
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Behaviour of NMDA and AMPA receptor-mediated miniature EPSCs at rat cortical neuron synapses identified by calcium imaging.
M. Umemiya, M. Senda, and T. H Murphy (1999)
J. Physiol. 521, 113-122
   Abstract »    Full Text »    PDF »
Correlation of Miniature Synaptic Activity and Evoked Release Probability in Cultures of Cortical Neurons.
O. Prange and T. H. Murphy (1999)
J. Neurosci. 19, 6427-6438
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Rapid Ca2+ Entry through Ca2+-Permeable AMPA/Kainate Channels Triggers Marked Intracellular Ca2+ Rises and Consequent Oxygen Radical Production.
S. G. Carriedo, H. Z. Yin, S. L. Sensi, and J. H. Weiss (1998)
J. Neurosci. 18, 7727-7738
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Calcium dynamics in single spines during coincident pre- and postsynaptic activity depend on relative timing of back-propagating action potentials and subthreshold excitatory postsynaptic potentials.
H. J. Koester and B. Sakmann (1998)
PNAS 95, 9596-9601
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Activation of Kv3.1 channels in neuronal spine-like structures may induce local potassium ion depletion.
L.-Y. Wang, L. Gan, T. M. Perney, I. Schwartz, and L. K. Kaczmarek (1998)
PNAS 95, 1882-1887
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Calcium Dynamics in Thorny Excrescences of CA3 Pyramidal Neurons.
D. B. Jaffe and T. H. Brown (1997)
J Neurophysiol 78, 10-18
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Presynaptic component of long-term potentiation visualized at individual hippocampal synapses.
A Malgaroli, A. Ting, B Wendland, A Bergamaschi, A Villa, R. Tsien, and R. Scheller (1995)
Science 268, 1624-1628
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Activation of Ca2+/Calmodulin-dependent Protein Kinase II within Post-synaptic Dendritic Spines of Cultured Hippocampal Neurons.
N. Inagaki, M. Nishizawa, N. Arimura, H. Yamamoto, Y. Takeuchi, E. Miyamoto, K. Kaibuchi, and M. Inagaki (2000)
J. Biol. Chem. 275, 27165-27171
   Abstract »    Full Text »    PDF »
Ultrastructural Correlates of Quantal Synaptic Function at Single CNS Synapses.
P. J. Mackenzie, G. S. Kenner, O. Prange, H. Shayan, M. Umemiya, and T. H. Murphy (1999)
J. Neurosci. 19, RC13
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