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Science 17 December 1993:
Vol. 262. no. 5141, pp. 1886 - 1889
DOI: 10.1126/science.8266080
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Articles

Science, Vol 262, Issue 5141, 1886-1889
Copyright © 1993 by American Association for the Advancement of Science

articles

Functional requirement of a site-specific ribose methylation in ribosomal RNA

K Sirum-Connolly and TL Mason

Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003.

The product of the PET56 nuclear gene of Saccharomyces cerevisiae was shown to be required for ribose methylation at a universally conserved nucleotide in the peptidyl transferase center of the mitochondrial large ribosomal RNA (21S rRNA). Cells reduced in this activity were deficient in formation of functional large subunits of the mitochondrial ribosome. The purified Pet56 protein catalyzed the site-specific formation of 2'-O-methylguanosine on in vitro transcripts of both mitochondrial 21S rRNA and Escherichia coli 23S rRNA. These results provide evidence for an essential modified nucleotide in rRNA.


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