Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 17 December 1993:
Vol. 262. no. 5141, pp. 1867 - 1870
DOI: 10.1126/science.8266074
Prev | Table of Contents | Next

Articles

Science, Vol 262, Issue 5141, 1867-1870
Copyright © 1993 by American Association for the Advancement of Science

articles

A functional recombinant myosin II lacking a regulatory light chain-binding site

TQ Uyeda and JA Spudich

Department of Biochemistry, Stanford University School of Medicine, CA 94305.

Myosin II, which converts the energy of adenosine triphosphate hydrolysis into the movement of actin filaments, is a hexamer of two heavy chains, two essential light chains, and two regulatory light chains (RLCs). Dictyostelium myosin II is known to be regulated in vitro by phosphorylation of the RLC. Cells in which the wild-type myosin II heavy chain was replaced with a recombinant form that lacks the binding site for RLC carried out cytokinesis and almost normal development, processes known to be dependent on functional myosin II. Characterization of the purified recombinant protein suggests that a complex of RLC and the RLC binding site of the heavy chain plays an inhibitory role for adenosine triphosphatase activity and a structural role for the movement of myosin along actin.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Myosin II Recruitment during Cytokinesis Independent of Centralspindlin-mediated Phosphorylation.
J. R. Beach and T. T. Egelhoff (2009)
J. Biol. Chem. 284, 27377-27383
   Abstract »    Full Text »    PDF »
Removal of the cardiac myosin regulatory light chain increases isometric force production.
K. Pant, J. Watt, M. Greenberg, M. Jones, D. Szczesna-Cordary, and J. R. Moore (2009)
FASEB J 23, 3571-3580
   Abstract »    Full Text »    PDF »
Direct evidence for roles of phosphorylated regulatory light chain of myosin II in furrow ingression during cytokinesis in HeLa cells.
S. Asano, K. Hamao, and H. Hosoya (2009)
Genes Cells 14, 555-568
   Abstract »    Full Text »    PDF »
Multiple Regulatory Steps Control Mammalian Nonmuscle Myosin II Assembly in Live Cells.
M. T. Breckenridge, N. G. Dulyaninova, and T. T. Egelhoff (2009)
Mol. Biol. Cell 20, 338-347
   Abstract »    Full Text »    PDF »
Actin-binding cleft closure in myosin II probed by site-directed spin labeling and pulsed EPR.
J. C. Klein, A. R. Burr, B. Svensson, D. J. Kennedy, J. Allingham, M. A. Titus, I. Rayment, and D. D. Thomas (2008)
PNAS 105, 12867-12872
   Abstract »    Full Text »    PDF »
Biological, Biochemical, and Kinetic Effects of Mutations of the Cardiomyopathy Loop of Dictyostelium Myosin II: IMPORTANCE OF ALA400.
X. Liu, S. Shu, M. Kovacs, and E. D. Korn (2005)
J. Biol. Chem. 280, 26974-26983
   Abstract »    Full Text »    PDF »
Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae.
J. Luo, E. A. Vallen, C. Dravis, S. E. Tcheperegine, B. Drees, and E. Bi (2004)
J. Cell Biol. 165, 843-855
   Abstract »    Full Text »    PDF »
Tail chimeras of Dictyostelium myosin II support cytokinesis and other myosin II activities but not full development.
S. Shu, X. Liu, C. A. Parent, T. Q. P. Uyeda, and E. D. Korn (2002)
J. Cell Sci. 115, 4237-4249
   Abstract »    Full Text »    PDF »
Cytokinesis in Eukaryotes.
D. A. Guertin, S. Trautmann, and D. McCollum (2002)
Microbiol. Mol. Biol. Rev. 66, 155-178
   Abstract »    Full Text »    PDF »
Molecular mechanism of myosin-II assembly at the division site in Schizosaccharomyces pombe.
F Motegi, K Nakano, and I Mabuchi (2000)
J. Cell Sci. 113, 1813-1825
   Abstract »    PDF »
Deletion of the Myopathy Loop of Dictyostelium Myosin II and Its Impact on Motor Functions.
N. Sasaki, H. Asukagawa, R. Yasuda, T. Hiratsuka, and K. Sutoh (1999)
J. Biol. Chem. 274, 37840-37844
   Abstract »    Full Text »    PDF »
Parameters That Specify the Timing of Cytokinesis.
C. B. Shuster and D. R. Burgess (1999)
J. Cell Biol. 146, 981-992
   Abstract »    Full Text »    PDF »
Cardiac myosin heavy chains lacking the light chain binding domain cause hypertrophic cardiomyopathy in mice.
R. E. Welikson, S. H. Buck, J. R. Patel, R. L. Moss, K. L. Vikstrom, S. M. Factor, S. Miyata, H. D. Weinberger, and L. A. Leinwand (1999)
Am J Physiol Heart Circ Physiol 276, H2148-H2158
   Abstract »    Full Text »    PDF »
In Vivo Observations of Myosin II Dynamics Support a Role in Rear Retraction.
P. A. Clow and J. G. McNally (1999)
Mol. Biol. Cell 10, 1309-1323
   Abstract »    Full Text »
Activation of Myosin Phosphatase Targeting Subunit by Mitosis-specific Phosphorylation.
G. Totsukawa, Y. Yamakita, S. Yamashiro, H. Hosoya, D. J. Hartshorne, and F. Matsumura (1999)
J. Cell Biol. 144, 735-744
   Abstract »    Full Text »    PDF »
Role of myosin II tail sequences in its function and localization at the cleavage furrow in Dictyostelium.
S Shu, R. Lee, J. LeBlanc-Straceski, and T. Uyeda (1999)
J. Cell Sci. 112, 2195-2201
   Abstract »    PDF »
Regulatory light chain mutations affect myosin motor function and kinetics.
B. Chaudoir, P. Kowalczyk, and R. Chisholm (1999)
J. Cell Sci. 112, 1611-1620
   Abstract »    PDF »
Filament structure as an essential factor for regulation of Dictyostelium myosin by regulatory light chain phosphorylation.
X. Liu, K. Ito, S. Morimoto, A. Hikkoshi-Iwane, T. Yanagida, and T. Q. P. Uyeda (1998)
PNAS 95, 14124-14129
   Abstract »    Full Text »    PDF »
Mutational Analysis of the Switch II Loop of Dictyostelium Myosin II.
N. Sasaki, T. Shimada, and K. Sutoh (1998)
J. Biol. Chem. 273, 20334-20340
   Abstract »    Full Text »    PDF »
Specific Localization of Serine 19 Phosphorylated Myosin II during Cell Locomotion and Mitosis of Cultured Cells.
F. Matsumura, S. Ono, Y. Yamakita, G. Totsukawa, and S. Yamashiro (1998)
J. Cell Biol. 140, 119-129
   Abstract »    Full Text »    PDF »
On the Role of Myosin-II in Cytokinesis: Division of Dictyostelium Cells under Adhesive and Nonadhesive Conditions.
J.-H. Zang, G. Cavet, J. H. Sabry, P. Wagner, S. L. Moores, and J. A. Spudich (1997)
Mol. Biol. Cell 8, 2617-2629
   Abstract »    Full Text »
Transport of Myosin II to the Equatorial Region without Its Own Motor Activity in Mitotic Dictyostelium Cells.
S. Yumura and T. Q.P. Uyeda (1997)
Mol. Biol. Cell 8, 2089-2099
   Abstract »    Full Text »
Substitution Mutations in the Myosin Essential Light Chain Lead to Reduced Actin-activated ATPase Activity Despite Stoichiometric Binding to the Heavy Chain.
G. Ho and RexL. Chisholm (1997)
J. Biol. Chem. 272, 4522-4527
   Abstract »    Full Text »    PDF »
The Structural Basis of the Myosin ATPase Activity.
I. Rayment and I. Rayment (1996)
J. Biol. Chem. 271, 15850-15853
   Full Text »    PDF »
Drosophila nonmuscle myosin II has multiple essential roles in imaginal disc and egg chamber morphogenesis.
K. Edwards and D. Kiehart (1996)
Development 122, 1499-1511
   Abstract »    PDF »
Both the Amino and Carboxyl Termini of Dictyostelium Myosin Essential Light Chain Are Required for Binding to Myosin Heavy Chain.
G. Ho, T.-L. L. Chen, and R. L. Chisholm (1995)
J. Biol. Chem. 270, 27977-27981
   Abstract »    Full Text »    PDF »
Cardiac V1 And V3 Myosins Differ in Their Hydrolytic and Mechanical Activities In Vitro.
P. VanBuren, D. E. Harris, N. R. Alpert, and D. M. Warshaw (1995)
Circ. Res. 77, 439-444
   Abstract »    Full Text »
Targeted disruption of the Dictyostelium myosin essential light chain gene produces cells defective in cytokinesis and morphogenesis.
T. Chen, P. Kowalczyk, G Ho, and R. Chisholm (1995)
J. Cell Sci. 108, 3207-3218
   Abstract »    PDF »
Myosin Structure and Function.
J.A. Spudich, J. Finer, B. Simmons, K. Ruppel, B. Patterson, and T. Uyeda (1995)
Cold Spring Harb Symp Quant Biol 60, 783-791
   Abstract »    PDF »
Regulation of myosin regulatory light chain phosphorylation via cyclic GMP during chemotaxis of Dictyostelium.
G. Liu and P. C. Newell (1994)
J. Cell Sci. 107, 1737-1743
   Abstract »    PDF »
Insertion or Deletion of a Single Residue in the Strut Sequence of Dictyostelium Myosin II Abolishes Strong Binding to Actin.
N. Sasaki, R. Ohkura, and K. Sutoh (2000)
J. Biol. Chem. 275, 38705-38709
   Abstract »    Full Text »    PDF »
Functional Characterization of Vertebrate Nonmuscle Myosin IIB Isoforms Using Dictyostelium Chimeric Myosin II.
M. Takahashi, K. Takahashi, Y. Hiratsuka, K. Uchida, A. Yamagishi, T. Q. P. Uyeda, and M. Yazawa (2001)
J. Biol. Chem. 276, 1034-1040
   Abstract »    Full Text »    PDF »
The Light Chain Binding Domain of Expressed Smooth Muscle Heavy Meromyosin Acts as a Mechanical Lever.
D. M. Warshaw, W. H. Guilford, Y. Freyzon, E. Krementsova, K. A. Palmiter, M. J. Tyska, J. E. Baker, and K. M. Trybus (2000)
J. Biol. Chem. 275, 37167-37172
   Abstract »    Full Text »    PDF »
Structure of Cdc4p, a Contractile Ring Protein Essential for Cytokinesis in Schizosaccharomyces pombe.
C. M. Slupsky, M. Desautels, T. Huebert, R. Zhao, S. M. Hemmingsen, and L. P. McIntosh (2001)
J. Biol. Chem. 276, 5943-5951
   Abstract »    Full Text »    PDF »
Chimeras of Dictyostelium myosin II head and neck domains with Acanthamoeba or chicken smooth muscle myosin II tail domain have greatly increased and unregulated actin-dependent MgATPase activity.
X. Liu, S. Shu, R. A. Yamashita, Y. Xu, and E. D. Korn (2000)
PNAS 97, 12553-12558
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)