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Science 10 December 1993:
Vol. 262. no. 5140, pp. 1728 - 1731
DOI: 10.1126/science.8259517
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Articles

Science, Vol 262, Issue 5140, 1728-1731
Copyright © 1993 by American Association for the Advancement of Science

articles

Initiation at closely spaced replication origins in a yeast chromosome

BJ Brewer and WL Fangman

Department of Genetics SK-50, University of Washington, Seattle 98195.

Replication of eukaryotic chromosomes involves initiation at origins spaced an average of 50 to 100 kilobase pairs. In yeast, potential origins can be recognized as autonomous replication sequences (ARSs) that allow maintenance of plasmids. However, there are more ARS elements than active chromosomal origins. The possibility was examined that close spacing of ARSs can lead to inactive origins. Two ARSs located 6.5 kilobase pairs apart can indeed interfere with each other. Replication is initiated from one or the other ARS with equal probability, but rarely (< 5%) from both ARSs on the same DNA molecule.


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