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Science 26 November 1993:
Vol. 262. no. 5138, pp. 1422 - 1425
DOI: 10.1126/science.262.5138.1422
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Articles

Single Molecules Observed by Near-Field Scanning Optical Microscopy

Eric Betzig 1 and Robert J. Chichester 1

1 AT&T Bell Laboratories, 600 Mountain Avenue, Murray Hill, NJ 07974

Individual carbocyanine dye molecules in a sub-monolayer spread have been imaged with near-field scanning optical microscopy. Molecules can be repeatedly detected and spatially localized (to simlgr/50 where lgr is the wavelength of light) with a sensitivity of at least 0.005 molecules/(Hz)1/2 and the orientation of each molecular dipole can be determined. This information is exploited to map the electric field distribution in the near-field aperture with molecular spatial resolution.

Submitted on September 15, 1993
Accepted on October 19, 1993


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Science. ISSN 0036-8075 (print), 1095-9203 (online)