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Science 26 November 1993: Vol. 262. no. 5138, pp. 1401 - 1407 DOI: 10.1126/science.8248779
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Articles
Science, Vol 262, Issue 5138, 1401-1407
Copyright © 1993 by American Association for the Advancement of Science
A switch between two-, three-, and four-stranded coiled coils in GCN4 leucine zipper mutants
PB Harbury,
T Zhang,
PS Kim,
and
T Alber
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.
Coiled-coil sequences in proteins consist of heptad repeats containing two characteristic hydrophobic positions. The role of these buried hydrophobic residues in determining the structures of coiled coils was investigated by studying mutants of the GCN4 leucine zipper. When sets of buried residues were altered, two-, three-, and four-helix structures were formed. The x-ray crystal structure of the tetramer revealed a parallel, four-stranded coiled coil. In the tetramer conformation, the local packing geometry of the two hydrophobic positions in the heptad repeat is reversed relative to that in the dimer. These studies demonstrate that conserved, buried residues in the GCN4 leucine zipper direct dimer formation. In contrast to proposals that the pattern of hydrophobic and polar amino acids in a protein sequence is sufficient to determine three-dimensional structure, the shapes of buried side chains in coiled coils are essential determinants of the global fold.
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J. Virol.
75, 7131-7141
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- Phosphorylation-dependent Regulation of Ryanodine Receptors: A Novel Role for Leucine/Isoleucine Zippers.
- S. O. Marx, S. Reiken, Y. Hisamatsu, M. Gaburjakova, J. Gaburjakova, Y.-M. Yang, N. Rosemblit, and A. R. Marks (2001)
J. Cell Biol.
153, 699-708
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- Rap1p Requires Gcr1p and Gcr2p Homodimers to Activate Ribosomal Protein and Glycolytic Genes, Respectively.
- S. J. Deminoff and G. M. Santangelo (2001)
Genetics
158, 133-143
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- In Vitro Assembly of Sindbis Virus Core-Like Particles from Cross-Linked Dimers of Truncated and Mutant Capsid Proteins.
- T. L. Tellinghuisen, R. Perera, and R. J. Kuhn (2001)
J. Virol.
75, 2810-2817
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- Adenovirus Type 5 Viral Particles Pseudotyped with Mutagenized Fiber Proteins Show Diminished Infectivity of Coxsackie B-Adenovirus Receptor-Bearing Cells.
- J. L. Jakubczak, M. L. Rollence, D. A. Stewart, J. D. Jafari, D. J. Von Seggern, G. R. Nemerow, S. C. Stevenson, and P. L. Hallenbeck (2001)
J. Virol.
75, 2972-2981
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- Breaking open a protein barrel.
- N. Kallenbach (2001)
PNAS
98, 2958-2960
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- Designed heterodimerizing leucine zippers with a ranger of pIs and stabilities up to 10-15 M.
- J. R. Moll, S. B. Ruvinov, I. Pastan, and C. Vinson (2001)
Protein Sci.
10, 649-655
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- Open-and-shut cases in coiled-coil assembly: {{alpha}}-sheets and {{alpha}}-cylinders.
- J. Walshaw and D. N. Woolfson (2001)
Protein Sci.
10, 668-673
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