Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 12 November 1993:
Vol. 262. no. 5136, pp. 1051 - 1054
DOI: 10.1126/science.8235622
Prev | Table of Contents | Next

Articles

Science, Vol 262, Issue 5136, 1051-1054
Copyright © 1993 by American Association for the Advancement of Science

articles

Release of active cytokinin by a beta-glucosidase localized to the maize root meristem

B Brzobohaty, I Moore, P Kristoffersen, L Bako, N Campos, J Schell, and K Palme

Max-Planck Institut fur Zuchtungsforschung, Koln Federal Republic of Germany.

A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60.1) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3-glucoside, releasing active cytokinin. Tobacco protoplasts that transiently expressed Zm-p60.1 could use the inactive cytokinin glucosides to initiate cell division. The ability of protoplasts to sustain growth in response to cytokinin glucosides persisted indefinitely after the likely disappearance of the expression vector. In the roots of maize seedlings, Zm-p60.1 was localized to the meristematic cells and may function in vivo to supply the developing maize embryo with active cytokinin.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Molecular Basis of Shoot Responses of Maize Seedlings to Trichoderma harzianum T22 Inoculation of the Root: A Proteomic Approach.
M. Shoresh and G. E. Harman (2008)
Plant Physiology 147, 2147-2163
   Abstract »    Full Text »    PDF »
Over-expression of a zeatin O-glucosylation gene in maize leads to growth retardation and tasselseed formation.
A. Pineda Rodo, N. Brugiere, R. Vankova, J. Malbeck, J. M. Olson, S. C. Haines, R. C. Martin, J. E. Habben, D. W. S. Mok, and M. C. Mok (2008)
J. Exp. Bot. 59, 2673-2686
   Abstract »    Full Text »    PDF »
Farnesylation Directs AtIPT3 Subcellular Localization and Modulates Cytokinin Biosynthesis in Arabidopsis.
A. Galichet, K. Hoyerova, M. Kaminek, and W. Gruissem (2008)
Plant Physiology 146, 1155-1164
   Abstract »    Full Text »    PDF »
Expression differences between normal and indeterminate1 maize suggest downstream targets of ID1, a floral transition regulator in maize.
V. Coneva, T. Zhu, and J. Colasanti (2007)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
Molecular and Structural Characterization of Hexameric beta-D-Glucosidases in Wheat and Rye.
M. Sue, K. Yamazaki, S. Yajima, T. Nomura, T. Matsukawa, H. Iwamura, and T. Miyamoto (2006)
Plant Physiology 141, 1237-1247
   Abstract »    Full Text »    PDF »
Functional Differentiation of Bundle Sheath and Mesophyll Maize Chloroplasts Determined by Comparative Proteomics.
W. Majeran, Y. Cai, Q. Sun, and K. J. van Wijk (2005)
PLANT CELL 17, 3111-3140
   Abstract »    Full Text »    PDF »
The {beta}-Glucuronidase Klotho Hydrolyzes and Activates the TRPV5 Channel.
Q. Chang, S. Hoefs, A. W. van der Kemp, C. N. Topala, R. J. Bindels, and J. G. Hoenderop (2005)
Science 310, 490-493
   Abstract »    Full Text »    PDF »
Characterization of Antirrhinum Petal Development and Identification of Target Genes of the Class B MADS Box Gene DEFICIENS.
M. Bey, K. Stuber, K. Fellenberg, Z. Schwarz-Sommer, H. Sommer, H. Saedler, and S. Zachgo (2004)
PLANT CELL 16, 3197-3215
   Abstract »    Full Text »    PDF »
N-Glucosylation of Cytokinins by Glycosyltransferases of Arabidopsis thaliana.
B. Hou, E.-K. Lim, G. S. Higgins, and D. J. Bowles (2004)
J. Biol. Chem. 279, 47822-47832
   Abstract »    Full Text »    PDF »
Arabidopsis CYP735A1 and CYP735A2 Encode Cytokinin Hydroxylases That Catalyze the Biosynthesis of trans-Zeatin.
K. Takei, T. Yamaya, and H. Sakakibara (2004)
J. Biol. Chem. 279, 41866-41872
   Abstract »    Full Text »    PDF »
The SENSITIVE TO FREEZING2 Gene, Required for Freezing Tolerance in Arabidopsis thaliana, Encodes a {beta}-Glucosidase.
G. Thorlby, N. Fourrier, and G. Warren (2004)
PLANT CELL 16, 2192-2203
   Abstract »    Full Text »    PDF »
Structural Determinants of Substrate Specificity in Family 1 {beta}-Glucosidases: NOVEL INSIGHTS FROM THE CRYSTAL STRUCTURE OF SORGHUM DHURRINASE-1, A PLANT {beta}-GLUCOSIDASE WITH STRICT SPECIFICITY, IN COMPLEX WITH ITS NATURAL SUBSTRATE.
L. Verdoucq, J. Moriniere, D. R. Bevan, A. Esen, A. Vasella, B. Henrissat, and M. Czjze (2004)
J. Biol. Chem. 279, 31796-31803
   Abstract »    Full Text »    PDF »
Furcatin Hydrolase from Viburnum furcatum Blume Is a Novel Disaccharide-specific Acuminosidase in Glycosyl Hydrolase Family 1.
Y. O. Ahn, M. Mizutani, H. Saino, and K. Sakata (2004)
J. Biol. Chem. 279, 23405-23414
   Abstract »    Full Text »    PDF »
Auxin regulation of cytokinin biosynthesis in Arabidopsis thaliana: A factor of potential importance for auxin-cytokinin-regulated development.
A. Nordstrom, P. Tarkowski, D. Tarkowska, R. Norbaek, C. Astot, K. Dolezal, and G. Sandberg (2004)
PNAS 101, 8039-8044
   Abstract »    Full Text »    PDF »
Klotho Is a Novel {beta}-Glucuronidase Capable of Hydrolyzing Steroid {beta}-Glucuronides.
O. Tohyama, A. Imura, A. Iwano, J.-N. Freund, B. Henrissat, T. Fujimori, and Y.-i. Nabeshima (2004)
J. Biol. Chem. 279, 9777-9784
   Abstract »    Full Text »    PDF »
The ER Body, a Novel Endoplasmic Reticulum-Derived Structure in Arabidopsis.
R. Matsushima, Y. Hayashi, K. Yamada, T. Shimada, M. Nishimura, and I. Hara-Nishimura (2003)
Plant Cell Physiol. 44, 661-666
   Abstract »    Full Text »    PDF »
Mutational and Structural Analysis of Aglycone Specificity in Maize and Sorghum {beta}-Glucosidases.
L. Verdoucq, M. Czjzek, J. Moriniere, D. R. Bevan, and A. Esen (2003)
J. Biol. Chem. 278, 25055-25062
   Abstract »    Full Text »    PDF »
The Identification of Candidate Genes for a Reverse Genetic Analysis of Development and Function in the Arabidopsis Gynoecium.
C. P. Scutt, M. Vinauger-Douard, C. Fourquin, J. Ailhas, N. Kuno, K. Uchida, T. Gaude, M. Furuya, and C. Dumas (2003)
Plant Physiology 132, 653-665
   Abstract »    Full Text »    PDF »
Dynamics of Endogenous Cytokinin Pools in Tobacco Seedlings: a Modelling Approach.
M. LEXA, T. GENKOV, J. MALBECK, I. MACHACKOVA, and B. BRZOBOHATY (2003)
Ann. Bot. 91, 585-597
   Abstract »    Full Text »    PDF »
Cytokinins. New Insights into a Classic Phytohormone.
G. Haberer and J. J. Kieber (2002)
Plant Physiology 128, 354-362
   Full Text »    PDF »
Insights into the Functional Architecture of the Catalytic Center of a Maize beta -Glucosidase Zm-p60.1.
J. Zouhar, J. Vevodova, J. Marek, J. Damborsky, X.-D. Su, and B. Brzobohaty (2001)
Plant Physiology 127, 973-985
   Abstract »    Full Text »    PDF »
Gene Expression Profiles during the Initial Phase of Salt Stress in Rice.
S. Kawasaki, C. Borchert, M. Deyholos, H. Wang, S. Brazille, K. Kawai, D. Galbraith, and H. J. Bohnert (2001)
PLANT CELL 13, 889-906
   Abstract »    Full Text »
Local and Systemic Changes in Squash Gene Expression in Response to Silverleaf Whitefly Feeding.
W. T. G. van de Ven, C. S. LeVesque, T. M. Perring, and L. L. Walling (2000)
PLANT CELL 12, 1409-1424
   Abstract »    Full Text »
Patterns of Protein Synthesis and Tolerance of Anoxia in Root Tips of Maize Seedlings Acclimated to a Low-Oxygen Environment, and Identification of Proteins by Mass Spectrometry.
W. W.P. Chang, L. Huang, M. Shen, C. Webster, A. L. Burlingame, and J. K.M. Roberts (2000)
Plant Physiology 122, 295-318
   Abstract »    Full Text »
A Specific beta -Glucosidase-Aggregating Factor Is Responsible for the beta -Glucosidase Null Phenotype in Maize.
A. Esen and D. J. Blanchard (2000)
Plant Physiology 122, 563-572
   Abstract »    Full Text »
A Gene Encoding the Cytokinin Enzyme Zeatin O-Xylosyltransferase of Phaseolus vulgaris.
R. C. Martin, M. C. Mok, and D. W.S. Mok (1999)
Plant Physiology 120, 553-558
   Abstract »    Full Text »
Isolation of a cytokinin gene, ZOG1, encoding zeatin O-glucosyltransferase from Phaseolus lunatus.
R. C. Martin, M. C. Mok, and D. W. S. Mok (1999)
PNAS 96, 284-289
   Abstract »    Full Text »    PDF »
Protein Changes in Response to Progressive Water Deficit in Maize . Quantitative Variation and Polypeptide Identification.
F. Riccardi, P. Gazeau, D. de Vienne, and M. Zivy (1998)
Plant Physiology 117, 1253-1263
   Abstract »    Full Text »
Structure and Expression of a Dhurrinase (beta -Glucosidase) from Sorghum.
M. Cicek and A. Esen (1998)
Plant Physiology 116, 1469-1478
   Abstract »    Full Text »
A transcription activation system for regulated gene expression in transgenic plants.
I. Moore, L. Galweiler, D. Grosskopf, J. Schell, and K. Palme (1998)
PNAS 95, 376-381
   Abstract »    Full Text »    PDF »
Biochemical and Molecular Characterization of a Barley Seed [IMAGE]-Glucosidase.
R. Leah, J. Kigel, I. Svendsen, and J. Mundy (1995)
J. Biol. Chem. 270, 15789-15797
   Abstract »    Full Text »    PDF »
The Aglycone Specificity-determining Sites Are Different in 2,4-Dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA)-glucosidase (Maize beta -Glucosidase) and Dhurrinase (Sorghum beta -Glucosidase).
M. Cicek, D. Blanchard, D. R. Bevan, and A. Esen (2000)
J. Biol. Chem. 275, 20002-20011
   Abstract »    Full Text »    PDF »
Identification of beta -Glucosidase Aggregating Factor (BGAF) and Mapping of BGAF Binding Regions on Maize beta -Glucosidase.
D. J. Blanchard, M. Cicek, J. Chen, and A. Esen (2001)
J. Biol. Chem. 276, 11895-11901
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)