Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Science Policy Alerts

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 22 October 1993:
Vol. 262. no. 5133, pp. 575 - 578
DOI: 10.1126/science.8211185
Prev | Table of Contents | Next

Articles

Science, Vol 262, Issue 5133, 575-578
Copyright © 1993 by American Association for the Advancement of Science

articles

Potentiation by the beta subunit of the ratio of the ionic current to the charge movement in the cardiac calcium channel

A Neely, X Wei, R Olcese, L Birnbaumer, and E Stefani

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030.

The voltage-activated rabbit cardiac calcium channel alpha 1 subunit was expressed in Xenopus oocytes. The charge movement of its voltage sensor was measured and related to the opening of the ion-conducting pore. The half-activation potential for charge movement was 35 millivolts more negative than that for pore opening. Coexpression of the cardiac calcium channel beta subunit reduced this difference without affecting charge movement. Thus, intramolecular coupling between the voltage sensor and the channel pore opening can be facilitated by a regulatory subunit.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mutations of Nonconserved Residues within the Calcium Channel {alpha}1-interaction Domain Inhibit {beta}-Subunit Potentiation.
G. Gonzalez-Gutierrez, E. Miranda-Laferte, D. Naranjo, P. Hidalgo, and A. Neely (2008)
J. Gen. Physiol. 132, 383-395
   Abstract »    Full Text »    PDF »
Influence of channel subunit composition on L-type Ca2+ current kinetics and cardiac wave stability.
V. Gudzenko, Y. Shiferaw, N. Savalli, R. Vyas, J. N. Weiss, and R. Olcese (2007)
Am J Physiol Heart Circ Physiol 293, H1805-H1815
   Abstract »    Full Text »    PDF »
Calcium and Arrhythmogenesis.
H. E. D. J. ter Keurs and P. A. Boyden (2007)
Physiol Rev 87, 457-506
   Abstract »    Full Text »    PDF »
The Src Homology 3 Domain of the beta-Subunit of Voltage-gated Calcium Channels Promotes Endocytosis via Dynamin Interaction.
G. Gonzalez-Gutierrez, E. Miranda-Laferte, A. Neely, and P. Hidalgo (2007)
J. Biol. Chem. 282, 2156-2162
   Abstract »    Full Text »    PDF »
The Role of Voltage-Gated Calcium Channels in Pancreatic {beta}-Cell Physiology and Pathophysiology.
S.-N. Yang and P.-O. Berggren (2006)
Endocr. Rev. 27, 621-676
   Abstract »    Full Text »    PDF »
The {alpha}1-beta-Subunit Interaction That Modulates Calcium Channel Activity Is Reversible and Requires a Competent {alpha}-Interaction Domain.
P. Hidalgo, G. Gonzalez-Gutierrez, J. Garcia-Olivares, and A. Neely (2006)
J. Biol. Chem. 281, 24104-24110
   Abstract »    Full Text »    PDF »
The importance of occupancy rather than affinity of CaV{beta} subunits for the calcium channel I-II linker in relation to calcium channel function.
A. J. Butcher, J. Leroy, M. W. Richards, W. S. Pratt, and A. C. Dolphin (2006)
J. Physiol. 574, 387-398
   Abstract »    Full Text »    PDF »
Regulation of Maximal Open Probability Is a Separable Function of Cav{beta} Subunit in L-type Ca2+ Channel, Dependent on NH2 Terminus of {alpha}1C (Cav1.2{alpha}).
N. Kanevsky and N. Dascal (2006)
J. Gen. Physiol. 128, 15-36
   Abstract »    Full Text »    PDF »
N-Cadherin Juxtamembrane Domain Modulates Voltage-Gated Ca2+ Current via RhoA GTPase and Rho-Associated Kinase.
G. Piccoli, U. Rutishauser, and J. L. Bruses (2004)
J. Neurosci. 24, 10918-10923
   Abstract »    Full Text »    PDF »
Uncoupling of Calcium Channel {alpha}1 and {beta} Subunits in Developing Neurons.
J. D. Spafford, J. van Minnen, P. Larsen, A. B. Smit, N. I. Syed, and G. W. Zamponi (2004)
J. Biol. Chem. 279, 41157-41167
   Abstract »    Full Text »    PDF »
The {alpha}2{delta} Auxiliary Subunit Reduces Affinity of {omega}-Conotoxins for Recombinant N-type (Cav2.2) Calcium Channels.
J. Mould, T. Yasuda, C. I. Schroeder, A. M. Beedle, C. J. Doering, G. W. Zamponi, D. J. Adams, and R. J. Lewis (2004)
J. Biol. Chem. 279, 34705-34714
   Abstract »    Full Text »    PDF »
Involvement of a Heptad Repeat in the Carboxyl Terminus of the Dihydropyridine Receptor {beta}1a Subunit in the Mechanism of Excitation-Contraction Coupling in Skeletal Muscle.
D. C. Sheridan, W. Cheng, L. Carbonneau, C. A. Ahern, and R. Coronado (2004)
Biophys. J. 87, 929-942
   Abstract »    Full Text »    PDF »
Folding of Active Calcium Channel {beta}1b -Subunit by Size-exclusion Chromatography and Its Role on Channel Function.
A. Neely, J. Garcia-Olivares, S. Voswinkel, H. Horstkott, and P. Hidalgo (2004)
J. Biol. Chem. 279, 21689-21694
   Abstract »    Full Text »    PDF »
Membrane-associated guanylate kinase-like properties of {beta}-subunits required for modulation of voltage-dependent Ca2+ channels.
S. X. Takahashi, J. Miriyala, and H. M. Colecraft (2004)
PNAS 101, 7193-7198
   Abstract »    Full Text »    PDF »
Overexpressed Cav{beta}3 Inhibits N-type (Cav2.2) Calcium Channel Currents through a Hyperpolarizing Shift of "Ultra-slow" and "Closed-state" Inactivation.
T. Yasuda, R. J. Lewis, and D. J. Adams (2004)
J. Gen. Physiol. 123, 401-416
   Abstract »    Full Text »    PDF »
G Protein Modulation of Voltage-Gated Calcium Channels.
A. C. Dolphin (2003)
Pharmacol. Rev. 55, 607-627
   Abstract »    Full Text »    PDF »
Ca2+-Dependent Excitation-Contraction Coupling Triggered by the Heterologous Cardiac/Brain DHPR {beta}2a-Subunit in Skeletal Myotubes.
D. C. Sheridan, L. Carbonneau, C. A. Ahern, P. Nataraj, and R. Coronado (2003)
Biophys. J. 85, 3739-3757
   Abstract »    Full Text »    PDF »
Modified Cardiovascular L-type Channels in Mice Lacking the Voltage-dependent Ca2+ Channel {beta}3 Subunit.
M. Murakami, H. Yamamura, T. Suzuki, M.-G. Kang, S. Ohya, A. Murakami, I. Miyoshi, H. Sasano, K. Muraki, T. Hano, et al. (2003)
J. Biol. Chem. 278, 43261-43267
   Abstract »    Full Text »    PDF »
Differential Modulation of Cardiac Ca2+ Channel Gating by {beta}-Subunits.
I. Dzhura and A. Neely (2003)
Biophys. J. 85, 274-289
   Abstract »    Full Text »    PDF »
Ca2+ Current and Charge Movements in Skeletal Myotubes Promoted by the {beta}-Subunit of the Dihydropyridine Receptor in the Absence of Ryanodine Receptor Type 1.
C. A. Ahern, David. C. Sheridan, W. Cheng, L. Mortenson, P. Nataraj, P. Allen, M. D. Waard, and R. Coronado (2003)
Biophys. J. 84, 942-959
   Abstract »    Full Text »    PDF »
Truncation of the Carboxyl Terminus of the DihydropyridineReceptor {beta}1a Subunit Promotes Ca2+ Dependent Excitation-Contraction Coupling in Skeletal Myotubes.
D. C. Sheridan, W. Cheng, C. A. Ahern, L. Mortenson, D. Alsammarae, P. Vallejo, and R. Coronado (2003)
Biophys. J. 84, 220-237
   Abstract »    Full Text »    PDF »
Increased Expression of alpha 1A Ca2+ Channel Currents Arising from Expanded Trinucleotide Repeats in Spinocerebellar Ataxia Type 6.
E. S. Piedras-Renteria, K. Watase, N. Harata, O. Zhuchenko, H. Y. Zoghbi, C. C. Lee, and R. W. Tsien (2001)
J. Neurosci. 21, 9185-9193
   Abstract »    Full Text »    PDF »
The {beta}2a Subunit Is a Molecular Groom for the Ca2+ Channel Inactivation Gate.
S. Restituito, T. Cens, C. Barrere, S. Geib, S. Galas, M. De Waard, and P. Charnet (2000)
J. Neurosci. 20, 9046-9052
   Abstract »    Full Text »    PDF »
Regulation of Cloned Cardiac L-type Calcium Channels by cGMP-dependent Protein Kinase.
L. H. Jiang, D. J. Gawler, N. Hodson, C. J. Milligan, H. A. Pearson, V. Porter, and D. Wray (2000)
J. Biol. Chem. 275, 6135-6143
   Abstract »    Full Text »    PDF »
Voltage-dependent facilitation of cardiac L-type Ca channels expressed in HEK-293 cells requires beta -subunit.
T. J. Kamp, H. Hu, and E. Marban (2000)
Am J Physiol Heart Circ Physiol 278, H126-H136
   Abstract »    Full Text »    PDF »
Ca channels in cardiac myocytes: structure and function in Ca influx and intracellular Ca release.
D. M Bers and E. Perez-Reyes (1999)
Cardiovasc Res 42, 339-360
   Abstract »    Full Text »    PDF »
Multiple Modulation Pathways of Calcium Channel Activity by a beta  Subunit. DIRECT EVIDENCE OF beta  SUBUNIT PARTICIPATION IN MEMBRANE TRAFFICKING OF THE alpha 1C SUBUNIT.
H. Yamaguchi, M. Hara, M. Strobeck, K. Fukasawa, A. Schwartz, and G. Varadi (1998)
J. Biol. Chem. 273, 19348-19356
   Abstract »    Full Text »    PDF »
Crucial Role of N Terminus in Function of Cardiac L-type Ca2+ Channel and Its Modulation by Protein Kinase C.
E. Shistik, T. Ivanina, Y. Blumenstein, and N. Dascal (1998)
J. Biol. Chem. 273, 17901-17909
   Abstract »    Full Text »    PDF »
L-type calcium channel expression depends on the differentiated state of vascular smooth muscle cells.
M. Gollasch, H. Haase, C. Ried, C. Lindschau, I. Morano, F. C. Luft, and H. Haller (1998)
FASEB J 12, 593-601
   Abstract »    Full Text »
Modulation of human neuronal alpha 1E-type calcium channel by alpha 2delta -subunit.
N. Qin, R. Olcese, E. Stefani, and L. Birnbaumer (1998)
Am J Physiol Cell Physiol 274, C1324-C1331
   Abstract »    Full Text »    PDF »
Unique regulatory properties of the type 2a Ca2+ channel beta  subunit caused by palmitoylation.
N. Qin, D. Platano, R. Olcese, J. L. Costantin, E. Stefani, and L. Birnbaumer (1998)
PNAS 95, 4690-4695
   Abstract »    Full Text »    PDF »
Differential Effects of Ca2+ Channel beta 1a and beta 2a Subunits on Complex Formation with alpha 1S and on Current Expression in tsA201 Cells.
B. Neuhuber, U. Gerster, J. Mitterdorfer, H. Glossmann, and B. E. Flucher (1998)
J. Biol. Chem. 273, 9110-9118
   Abstract »    Full Text »    PDF »
A Mutation Affecting Dihydropyridine-Sensitive Current Levels and Activation Kinetics in Drosophila Muscle and Mammalian Heart Calcium Channels.
D. Ren, H. Xu, D. F. Eberl, M. Chopra, and L. M. Hall (1998)
J. Neurosci. 18, 2335-2341
   Abstract »    Full Text »    PDF »
Molecular Studies on the Voltage Dependence of Dihydropyridine Action on L-type Ca2+ Channels. CRITICAL INVOLVEMENT OF TYROSINE RESIDUES IN MOTIF IIIS6 AND IVS6.
I. Bodi, H. Yamaguchi, M. Hara, M. He, A. Schwartz, and G. Varadi (1997)
J. Biol. Chem. 272, 24952-24960
   Abstract »    Full Text »    PDF »
Dissection of Functional Domains of the Voltage-Dependent Ca2+ Channel alpha 2delta Subunit.
R. Felix, C. A. Gurnett, M. De Waard, and K. P. Campbell (1997)
J. Neurosci. 17, 6884-6891
   Abstract »    Full Text »    PDF »
Voltage-controlled gating in a large conductance Ca2+-sensitive K+channel (hslo).
E. Stefani, M. Ottolia, F. Noceti, R. Olcese, M. Wallner, R. Latorre, and L. Toro (1997)
PNAS 94, 5427-5431
   Abstract »    Full Text »    PDF »
Feedback inhibition of Ca2+ channels by Ca2+ depends on a short sequence of the C terminus that does not include the Ca2+-binding function of a motif with similarity to Ca2+-binding domains.
J. Zhou, R. Olcese, N. Qin, F. Noceti, L. Birnbaumer, and E. Stefani (1997)
PNAS 94, 2301-2305
   Abstract »    Full Text »    PDF »
A Xenopus oocyte beta  subunit: Evidence for a role in the assembly/expression of voltage-gated calcium channels that is separate from its role as a regulatory subunit.
E. Tareilus, M. Roux, N. Qin, R. Olcese, J. Zhou, E. Stefani, and L. Birnbaumer (1997)
PNAS 94, 1703-1708
   Abstract »    Full Text »    PDF »
The Intracellular Loop between Domains I and II of the B-Type Calcium Channel Confers Aspects of G-Protein Sensitivity to the E-Type Calcium Channel.
K. M. Page, G. J. Stephens, N. S. Berrow, and A. C. Dolphin (1997)
J. Neurosci. 17, 1330-1338
   Abstract »    Full Text »    PDF »
A Drosophila Calcium Channel alpha 1 Subunit Gene Maps to a Genetic Locus Associated with Behavioral and Visual Defects.
L. A. Smith, X. Wang, A. A. Peixoto, E. K. Neumann, L. M. Hall, and J. C. Hall (1996)
J. Neurosci. 16, 7868-7879
   Abstract »    Full Text »    PDF »
Absence of the beta  subunit (cchb1) of the skeletal muscle dihydropyridine receptor alters expression of the alpha 1 subunit and eliminates excitation-contraction coupling.
R. G. Gregg, A. Messing, C. Strube, M. Beurg, R. Moss, M. Behan, M. Sukhareva, S. Haynes, J. A. Powell, R. Coronado, et al. (1996)
PNAS 93, 13961-13966
   Abstract »    Full Text »    PDF »
Roles of a Membrane-localized beta Subunit in the Formation and Targeting of Functional L-type Ca[IMAGE] Channels.
A. J. Chien, X. Zhao, R. E. Shirokov, T. S. Puri, C. F. Chang, D. Sun, E. Rios, and M. M. Hosey (1995)
J. Biol. Chem. 270, 30036-30044
   Abstract »    Full Text »    PDF »
Molecular Determinants of Cardiac Ca[IMAGE] Channel Pharmacology.
X. Wei, S. Pan, W. Lang, H. Kim, T. Schneider, E. Perez-Reyes, and L. Birnbaumer (1995)
J. Biol. Chem. 270, 27106-27111
   Abstract »    Full Text »    PDF »
Association of Native Ca[IMAGE] Channel [IMAGE] Subunits with the [IMAGE][IMAGE] Subunit Interaction Domain.
D. R. Witcher, M. D. Waard, H. Liu, M. Pragnell, and K. P. Campbell (1995)
J. Biol. Chem. 270, 18088-18093
   Abstract »    Full Text »    PDF »
Properties of the [IMAGE][IMAGE]-[IMAGE] Anchoring Site in Voltage-dependent Ca[IMAGE] Channels.
M. D. Waard, D. R. Witcher, M. Pragnell, H. Liu, and K. P. Campbell (1995)
J. Biol. Chem. 270, 12056-12064
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)