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Science 22 October 1993:
Vol. 262. no. 5133, pp. 573 - 575
DOI: 10.1126/science.8211184
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Articles

Science, Vol 262, Issue 5133, 573-575
Copyright © 1993 by American Association for the Advancement of Science

articles

U2AF homolog required for splicing in vivo

J Potashkin, K Naik, and K Wentz-Hunter

Department of Pharmacology and Molecular Biology, University of Health Sciences, Chicago Medical School, IL 60064.

Several fission yeast temperature-sensitive mutants defective in pre-mRNA processing (prp- mutants) at the nonpermissive temperature have been identified. Here, the prp2+ gene has been cloned by its ability to complement the temperature-sensitive growth defect of a prp2- mutant. The gene also corrects the pre-mRNA splicing defect of prp2- mutants and encodes a 59-kilodalton polypeptide (PRP2). A molecular characterization indicates that PRP2 is a previously uncharacterized yeast splicing factor with extensive similarity to the mammalian splicing factor U2AF65. Thus, this study provides evidence that a U2AF homolog participates in RNA processing in vivo.


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