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Science 22 October 1993:
Vol. 262. no. 5133, pp. 569 - 573
DOI: 10.1126/science.7692602
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Articles

Science, Vol 262, Issue 5133, 569-573
Copyright © 1993 by American Association for the Advancement of Science

articles

The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability

R Kanaar, SE Roche, EL Beall, MR Green, and DC Rio

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

The large subunit of the human pre-messenger RNA splicing factor U2 small nuclear ribonucleoprotein auxiliary factor (hU2AF65) is required for spliceosome assembly in vitro. A complementary DNA clone encoding the large subunit of Drosophila U2AF (dU2AF50) has been isolated. The dU2AF50 protein is closely related to its mammalian counterpart and contains three carboxyl-terminal ribonucleoprotein consensus sequence RNA binding domains and an amino-terminal arginine- and serine-rich (R/S) domain. Recombinant dU2AF50 protein complements mammalian splicing extracts depleted of U2AF activity. Germline transformation of Drosophila with the dU2AF50 complementary DNA rescues a lethal mutation, establishing that the dU2AF50 gene is essential for viability. R/S domains have been found in numerous metazoan splicing factors, but their function is unknown. The mutation in Drosophila U2AF will allow in vivo analysis of a conserved R/S domain-containing general splicing factor.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)