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Science 17 September 1993:
Vol. 261. no. 5128, pp. 1581 - 1584
DOI: 10.1126/science.8372352
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Articles

Science, Vol 261, Issue 5128, 1581-1584
Copyright © 1993 by American Association for the Advancement of Science

articles

Thymic selection of cytotoxic T cells independent of CD8 alpha-Lck association

IT Chan, A Limmer, MC Louie, ED Bullock, WP Fung-Leung, TW Mak, and DY Loh

Howard Hughes Medical Institute, Department of Medicine, Genetics, and Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.

The CD8 alpha cytoplasmic domain associates with p56lck, a nonreceptor protein-tyrosine kinase. The biological relevance of CD8 alpha-Lck association in T cell development was tested with transgenic mice generated to express a CD8 alpha molecule with two amino acid substitutions in its cytoplasmic domain, which abolishes the association of CD8 alpha with Lck. The CD8 alpha mutant was analyzed in a CD8-/- background and in the context of the transgenic 2C T cell receptor. The development and function of CD8+ T cells in these mice were apparently normal. Thus, CD8 alpha-Lck association is not necessary for positive selection, negative selection, or CD8-dependent cytotoxic function.


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