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Science 17 September 1993:
Vol. 261. no. 5128, pp. 1574 - 1577
DOI: 10.1126/science.7690496
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Articles

Science, Vol 261, Issue 5128, 1574-1577
Copyright © 1993 by American Association for the Advancement of Science

articles

Major groove accessibility of RNA

KM Weeks and DM Crothers

Department of Chemistry, Yale University, New Haven, CT 06511.

Chemical acylation experiments showed that the RNA major groove, often assumed to be too deep and narrow to permit recognition interactions, is accessible at duplex termini. Reactivity extended further into the helix in the 5' than in the 3' direction. Asymmetric and large loops between helices uncoupled them, which yielded both enhanced reactivity at terminal base pairs and weaker stabilization enthalpy compared to that in small loops or symmetric loops of the same size. Uncoupled helices have effective helix ends with accessible major grooves; such motifs are attractive contributors to protein recognition, tertiary folding, and catalysis.


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