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Science 13 August 1993:
Vol. 261. no. 5123, pp. 895 - 897
DOI: 10.1126/science.261.5123.895
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Articles

Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip

D. Jed Harrison 1, Karl Fluri 1, Kurt Seiler 1, Zhonghui Fan 1, Carlo S. Effenhauser 2, and Andreas Manz 2

1 Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2
2 Corporate Analytical Research, Ciba-Geigy Ltd., CH4002 Basel, Switzerland

Micromachining technology was used to prepare chemical analysis systems on glass chips (1 centimeter by 2 centimeters or larger) that utilize electroosmotic pumping to drive fluid flow and electrophoretic separation to distinguish sample components. Capillaries 1 to 10 centimeters long etched in the glass (cross section, 10 micrometers by 30 micrometers) allow for capillary electrophoresis-based separations of amino acids with up to 75,000 theoretical plates in about 15 seconds, and separations of about 600 plates can be effected within 4 seconds. Sample treatment steps within a manifold of intersecting capillaries were demonstrated for a simple sample dilution process. Manipulation of the applied voltages controlled the directions of fluid flow within the manifold. The principles demonstrated in this study can be used to develop a miniaturized system for sample handling and separation with no moving parts.

Submitted on March 10, 1993
Accepted on June 8, 1993


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