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Science 6 August 1993:
Vol. 261. no. 5122, pp. 759 - 762
DOI: 10.1126/science.8342041
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Articles

Science, Vol 261, Issue 5122, 759-762
Copyright © 1993 by American Association for the Advancement of Science

articles

DNA melting on yeast RNA polymerase II promoters

C Giardina and JT Lis

Section of Biochemistry, Molecular, and Cell Biology, Cornell University, Ithaca, NY 14853.

Transcription-dependent DNA melting on the yeast GAL1 and GAL10 promoters was found to be more closely correlated with the TATA box than the transcription start site. On both these genes, melting begins about 20 base pairs downstream of the TATA box. Physical and genetic analyses suggest that RNA polymerase II associates with this region. Thus, the distance between promoter melting and the TATA box in yeast may be similar to that in higher eukaryotes, even though transcription initiates in a region about 10 to 90 base pairs farther downstream in yeast.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)