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Science 30 July 1993:
Vol. 261. no. 5121, pp. 598 - 600
DOI: 10.1126/science.8342022
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Articles

Science, Vol 261, Issue 5121, 598-600
Copyright © 1993 by American Association for the Advancement of Science

articles

Greater susceptibility to mutations in lagging strand of DNA replication in Escherichia coli than in leading strand

X Veaute and RP Fuchs

Unite Propre de Recherche Cancerogenese et Mutagenese Moleculaire et Structurale, Institut de Biologie Moleculaire et Cellulaire Centre National de la Recherche Scientifique, Strasbourg, France.

Models of DNA replication in Escherichia coli involve an asymmetric DNA polymerase complex that replicates concurrently the leading and the lagging strands of double-stranded DNA. The effect of asymmetry on mutagenesis was tested with pairs of plasmids containing the unidirectional ColE1 origin of replication and a single lesion located in the leading or lagging strand. The lesion used was the covalent adduct that the chemical carcinogen N-2-acetylaminofluorene (AAF) forms with the C-8 position of guanine. Whether SOS was induced or not, mutations arose at about a 20-fold higher frequency when the AAF adduct was located in the lagging strand than when in the leading strand.


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