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Science 23 July 1993:
Vol. 261. no. 5120, pp. 480 - 483
DOI: 10.1126/science.7687382
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Articles

Science, Vol 261, Issue 5120, 480-483
Copyright © 1993 by American Association for the Advancement of Science

articles

Calcium-dependent heparin-like ligands for L-selectin in nonlymphoid endothelial cells

KE Norgard-Sumnicht, NM Varki, and A Varki

Glycobiology Program, UCSD Cancer Center.

L-Selectin is a calcium-dependent mammalian lectin that mediates lymphocyte trafficking by recognizing sialylated ligands on high endothelial venules in lymph nodes. Although L-selectin probably mediates neutrophil extravasation into nonlymphoid tissues, no corresponding ligand has been characterized. Staining of cultured endothelial cells with an L-selectin chimera (LS-Rg) showed an internal pool of ligands. Metabolic labeling with sulfur-35-labeled sulfate revealed heparin lyase-sensitive ligands that bound LS-Rg in a calcium-dependent, sialic acid-independent manner. A fraction of commercial heparin bound to LS-Rg and LS-Rg bound to heparin-agarose, both in a calcium-dependent manner. Thus, L-selectin recognizes endothelial heparin-like chains, which could be physiological ligands mediating leucocyte trafficking.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)