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Science 23 July 1993:
Vol. 261. no. 5120, pp. 463 - 466
DOI: 10.1126/science.8332911
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Articles

Science, Vol 261, Issue 5120, 463-466
Copyright © 1993 by American Association for the Advancement of Science

articles

Transcription factor TFIIB sites important for interaction with promoter-bound TFIID

S Yamashita, K Hisatake, T Kokubo, K Doi, RG Roeder, M Horikoshi, and Y Nakatani

National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, MD 20892.

Transcription initiation factor TFIIB recruits RNA polymerase II to the promoter subsequent to interaction with a preformed TFIID-promoter complex. The domains of TFIIB required for binding to the TFIID-promoter complex and for transcription initiation have been determined. The carboxyl-terminal two-thirds of TFIIB, which contains two direct repeats and two basic residue repeats, is sufficient for interaction with the TFIID-promoter complex. An extra 84-residue amino-terminal region, with no obvious known structural motifs, is required for basal transcription activity. Basic residues within the second basic repeat of TFIIB are necessary for stable interaction with the TFIID-promoter complex, whereas the basic character of the first basic repeat is not. Functional roles of other potential structural motifs are discussed in light of the present study.


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