Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 2 July 1993:
Vol. 261. no. 5117, pp. 93 - 95
DOI: 10.1126/science.7686307
Prev | Table of Contents | Next

Articles

Science, Vol 261, Issue 5117, 93-95
Copyright © 1993 by American Association for the Advancement of Science

articles

Interleukin-7: a cofactor for V(D)J rearrangement of the T cell receptor beta gene

K Muegge, MP Vila, and SK Durum

Biological Carcinogenesis and Development Program, Program Resources Inc./Dyncorp, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702.

The diversity of the T cell receptor repertoire is generated by rearrangement of gene elements in immature thymocytes. To identify a thymic signal that induces this rearrangement, a variety of agents were tested for their ability to induce rearrangement of the T cell receptor beta gene in suspensions of thymocytes from mouse embryos at day 14 of gestation. Of 16 agents tested, only interleukin-7 (IL-7) induced V(D)J gene rearrangement and sustained expression of the RAG-1 and RAG-2 genes, which are known to control rearrangement. These data implicate IL-7, a cytokine that is abundantly expressed in embryonic thymus, in driving gene rearrangement during early T cell development.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Use of interleukin 7 receptor-{alpha} knockout donor cells demonstrates the lymphoid independence of dendritic cells.
S. Takeuchi and S. I. Katz (2006)
Blood 107, 184-186
   Abstract »    Full Text »    PDF »
Up-Regulation of IL-7, Stromal-Derived Factor-1{alpha}, Thymus-Expressed Chemokine, and Secondary Lymphoid Tissue Chemokine Gene Expression in the Stromal Cells in Response to Thymocyte Depletion: Implication for Thymus Reconstitution.
I. Zubkova, H. Mostowski, and M. Zaitseva (2005)
J. Immunol. 175, 2321-2330
   Abstract »    Full Text »    PDF »
The Many Faces of IL-7: From Lymphopoiesis to Peripheral T Cell Maintenance.
T. J. Fry and C. L. Mackall (2005)
J. Immunol. 174, 6571-6576
   Abstract »    Full Text »    PDF »
T Cell Receptor Revision Does Not Solely Target Recent Thymic Emigrants.
C. J. Cooper, M. T. Orr, C. J. McMahan, and P. J. Fink (2003)
J. Immunol. 171, 226-233
   Abstract »    Full Text »    PDF »
Effects of exogenous interleukin-7 on human thymus function.
Y. Okamoto, D. C. Douek, R. D. McFarland, and R. A. Koup (2002)
Blood 99, 2851-2858
   Abstract »    Full Text »    PDF »
Radiosensitivity of thymic interleukin-7 production and thymopoiesis after bone marrow transplantation.
B. Chung, L. Barbara-Burnham, L. Barsky, and K. Weinberg (2001)
Blood 98, 1601-1606
   Abstract »    Full Text »    PDF »
Human Recent Thymic Emigrants-Identification, Expansion, And Survival Characteristics.
J. Hassan and D. J. Reen (2001)
J. Immunol. 167, 1970-1976
   Abstract »    Full Text »    PDF »
Control of chromatin accessibility for V(D)J recombination by interleukin-7.
J. Huang and K. Muegge (2001)
J. Leukoc. Biol. 69, 907-911
   Abstract »    Full Text »    PDF »
Control of T Cell Development In Vivo by Subdomains Within the IL-7 Receptor {{alpha}}-Chain Cytoplasmic Tail.
B. O. Porter, P. Scibelli, and T. R. Malek (2001)
J. Immunol. 166, 262-269
   Abstract »    Full Text »    PDF »
A partial deficiency of interleukin-7Ralpha is sufficient to abrogate T-cell development and cause severe combined immunodeficiency.
C. M. Roifman, J. Zhang, D. Chitayat, and N. Sharfe (2000)
Blood 96, 2803-2807
   Abstract »    Full Text »    PDF »
Neuroendocrine Control of Thymus Physiology.
W. Savino and M. Dardenne (2000)
Endocr. Rev. 21, 412-443
   Abstract »    Full Text »    PDF »
Murine Pro-B Cells Require IL-7 and Its Receptor Complex to Up-Regulate IL-7R{alpha}, Terminal Deoxynucleotidyltransferase, and c{micro} Expression.
C. Wei, R. Zeff, and I. Goldschneider (2000)
J. Immunol. 164, 1961-1970
   Abstract »    Full Text »    PDF »
Differential Regulation of Constitutive Major Histocompatibility Complex Class I Expression in T and B Lymphocytes.
C.-K. Lee, R. Gimeno, and D. E. Levy (1999)
J. Exp. Med. 190, 1451-1464
   Abstract »    Full Text »    PDF »
Defective Development of {gamma}/{delta} T Cells in Interleukin 7 Receptor-Deficient Mice Is Due to Impaired Expression of T Cell Receptor {gamma} Genes.
J. Kang, M. Coles, and D. H. Raulet (1999)
J. Exp. Med. 190, 973-982
   Abstract »    Full Text »    PDF »
Distinct Effects of Jak3 Signaling on {alpha}{beta} and {gamma}{delta} Thymocyte Development.
E. E. Eynon, F. Livak, K. Kuida, D. G. Schatz, and R. A. Flavell (1999)
J. Immunol. 162, 1448-1459
   Abstract »    Full Text »    PDF »
Role of Bcl-2 in {alpha}{beta} T Cell Development in Mice Deficient in the Common Cytokine Receptor {gamma}-Chain: The Requirement for Bcl-2 Differs Depending on the TCR/MHC Affinity.
H. Nakajima and W. J. Leonard (1999)
J. Immunol. 162, 782-790
   Abstract »    Full Text »    PDF »
Interleukin 7 Receptor Control of T Cell Receptor {gamma} Gene Rearrangement: Role of Receptor-associated Chains and Locus Accessibility.
S. K. Durum, S. Candeias, H. Nakajima, W. J. Leonard, A. M. Baird, L. J. Berg, and K. Muegge (1998)
J. Exp. Med. 188, 2233-2241
   Abstract »    Full Text »    PDF »
CD16 Cross-Linking Blocks Rearrangement of the TCR{beta} Locus and Development of {alpha}{beta} T Cells and Induces Development of NK Cells from Thymic Progenitors.
S. K. Durum, C.-K. Lee, T. M. Geiman, W. J. Murphy, and K. Muegge (1998)
J. Immunol. 161, 3325-3329
   Abstract »    Full Text »    PDF »
Expression of Recombination Activating Genes in Germinal Center B Cells: Involvement of Interleukin 7 (IL-7) and the IL-7 Receptor.
M. Hikida, Y. Nakayama, Y. Yamashita, Y. Kumazawa, S.-I. Nishikawa, and H. Ohmori (1998)
J. Exp. Med. 188, 365-372
   Abstract »    Full Text »    PDF »
Loss of Function of the Homeobox Gene Hoxa-9 Perturbs Early T-Cell Development and Induces Apoptosis in Primitive Thymocytes.
D. J. Izon, S. Rozenfeld, S. T. Fong, L. Komuves, C. Largman, and H. J. Lawrence (1998)
Blood 92, 383-393
   Abstract »    Full Text »    PDF »
The Trophic Action of IL-7 on Pro-T Cells: Inhibition of Apoptosis of Pro-T1, -T2, and -T3 Cells Correlates with Bcl-2 and Bax Levels and Is Independent of Fas and p53 Pathways.
K. Kim, C.-k. Lee, T. J. Sayers, K. Muegge, and S. K. Durum (1998)
J. Immunol. 160, 5735-5741
   Abstract »    Full Text »    PDF »
The V-J Recombination of T Cell Receptor-{gamma} Genes Is Blocked in Interleukin-7 Receptor-deficient Mice.
K. Maki, S. Sunaga, and K. Ikuta (1996)
J. Exp. Med. 184, 2423-2428
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)