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Science 11 June 1993:
Vol. 260. no. 5114, pp. 1658 - 1661
DOI: 10.1126/science.8503013
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Articles

Science, Vol 260, Issue 5114, 1658-1661
Copyright © 1993 by American Association for the Advancement of Science

articles

Complexes of Ras.GTP with Raf-1 and mitogen-activated protein kinase kinase

SA Moodie, BM Willumsen, MJ Weber, and A Wolfman

Department of Cell Biology, Cleveland Clinic Foundation, OH 44106.

The guanosine triphosphate (GTP)-binding protein Ras functions in regulating growth and differentiation; however, little is known about the protein interactions that bring about its biological activity. Wild-type Ras or mutant forms of Ras were covalently attached to an insoluble matrix and then used to examine the interaction of signaling proteins with Ras. Forms of Ras activated either by mutation (Gly12Val) or by binding of the GTP analog, guanylyl-imidodiphosphate (GMP-PNP) interacted specifically with Raf-1 whereas an effector domain mutant, Ile36Ala, failed to interact with Raf-1. Mitogen-activated protein kinase (MAP kinase) activity was only associated with activated forms of Ras. The specific interaction of activated Ras with active MAP kinase kinase (MAPKK) was confirmed by direct assays. Thus the forming of complexes containing MAPKK activity and Raf-1 protein are dependent upon the activity of Ras.


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   Abstract »    Full Text »    PDF »
The hsp90-binding Antibiotic Geldanamycin Decreases Raf Levels and Epidermal Growth Factor Signaling without Disrupting Formation of Signaling Complexes or Reducing the Specific Enzymatic Activity of Raf Kinase.
L. F. Stancato, A. M. Silverstein, J. K. Owens-Grillo, Y.-H. Chow, R. Jove, and W. B. Pratt (1997)
J. Biol. Chem. 272, 4013-4020
   Abstract »    Full Text »    PDF »
Differential Regulation of Raf-1, A-Raf, and B-Raf by Oncogenic Ras and Tyrosine Kinases.
R. Marais, Y. Light, H. F. Paterson, C. S. Mason, and C. J. Marshall (1997)
J. Biol. Chem. 272, 4378-4383
   Abstract »    Full Text »    PDF »
Actin-biding Protein-280 Binds the Stress-activated Protein Kinase (SAPK) Activator SEK-1 and Is Required for Tumor Necrosis Factor-alpha Activation of SAPK in Melanoma Cells.
A. Marti, Z. Luo, C. Cunningham, Y. Ohta, J. Hartwig, T. P. Stossel, J. M. Kyriakis, and J. Avruch (1997)
J. Biol. Chem. 272, 2620-2628
   Abstract »    Full Text »    PDF »
Sequential Modification of Serines 621and 624in the Raf-1 Carboxyl Terminus Produces Alterations in Its Electrophoretic Mobility.
A. F. Ferrier, M. Lee, W. B. Anderson, G. Benvenuto, D. K. Morrison, D. R. Lowy, and J. E. DeClue (1997)
J. Biol. Chem. 272, 2136-2142
   Abstract »    Full Text »    PDF »
The Drosophila 14-3-3 protein Leonardo enhances Torso signaling through D-Raf in a Ras 1-dependent manner.
W Li, E. Skoulakis, R. Davis, and N Perrimon (1997)
Development 124, 4163-4171
   Abstract »    PDF »
Suppression of Nerve Growth Factor-induced Neuronal Differentiation of PC12 Cells. N-ACETYLCYSTEINE UNCOUPLES THE SIGNAL TRANSDUCTION FROM Ras TO THE MITOGEN-ACTIVATED PROTEIN KINASE CASCADE.
H. Kamata, C. Tanaka, H. Yagisawa, S. Matsuda, Y. Gotoh, E. Nishida, and H. Hirata (1996)
J. Biol. Chem. 271, 33018-33025
   Abstract »    Full Text »    PDF »
Molecular Cloning and Characterization of a Novel Protein Kinase with a Catalytic Domain Homologous to Mitogen-activated Protein Kinase Kinase Kinase.
X. S. Wang, K. Diener, D. Jannuzzi, D. Trollinger, T.-H. Tan, H. Lichenstein, M. Zukowski, and Z. Yao (1996)
J. Biol. Chem. 271, 31607-31611
   Abstract »    Full Text »    PDF »
Insulin Signaling and Its Regulation of System A Amino Acid Uptake in Cultured Rat Vascular Smooth Muscle Cells.
T. Obata, A. Kashiwagi, H. Maegawa, Y. Nishio, S. Ugi, H. Hidaka, and R. Kikkawa (1996)
Circ. Res. 79, 1167-1176
   Abstract »    Full Text »
KSR modulates signal propagation within the MAPK cascade..
M Therrien, N R Michaud, G M Rubin, and D K Morrison (1996)
Genes & Dev. 10, 2684-2695
   Abstract »    PDF »
Identification of a Novel Guanine Nucleotide Exchange Factor for the Rho GTPase.
M. J. Hart, S. Sharma, N. elMasry, R.-G. Qiu, P. McCabe, P. Polakis, and G. Bollag (1996)
J. Biol. Chem. 271, 25452-25458
   Abstract »    Full Text »    PDF »
The Renaturable 69-and 63-kDa Protein Kinases That Undergo Rapid Activation in Chemoattractant-stimulated Guinea Pig Neutrophils Are p21-Activated Kinases.
J. Ding, U. G. Knaus, J. P. Lian, G. M. Bokoch, and J. A. Badwey (1996)
J. Biol. Chem. 271, 24869-24873
   Abstract »    Full Text »    PDF »
Nerve Growth Factor-mediated Activation of the Mitogen-activated Protein (MAP) Kinase Cascade Involves a Signaling Complex Containing B-Raf and HSP90.
R. K. Jaiswal, E. Weissinger, W. Kolch, and G. E. Landreth (1996)
J. Biol. Chem. 271, 23626-23629
   Abstract »    Full Text »    PDF »
Different Effects of Various Phospholipids on Ki-Ras-, Ha-Ras-, and Rap1B-induced B-Raf Activation.
S. Kuroda, T. Ohtsuka, B. Yamamori, K. Fukui, K. Shimizu, and Y. Takai (1996)
J. Biol. Chem. 271, 14680-14683
   Abstract »    Full Text »    PDF »
Identification of an Essential Signaling Cascade for Mitogen-activated Protein Kinase Activation by Angiotensin II in Cultured Rat Vascular Smooth Muscle Cells. POSSIBLE REQUIREMENT OF Gq-MEDIATED p21ras ACTIVATION COUPLED TO A Ca2+/CALMODULIN-SENSITIVE TYROSINE KINASE.
S. Eguchi, T. Matsumoto, E. D. Motley, H. Utsunomiya, and T. Inagami (1996)
J. Biol. Chem. 271, 14169-14175
   Abstract »    Full Text »    PDF »
Localization of Epidermal Growth Factor-stimulated Ras/Raf-1 Interaction to Caveolae Membrane.
C. Mineo, G. L. James, E. J. Smart, and R. G. W. Anderson (1996)
J. Biol. Chem. 271, 11930-11935
   Abstract »    Full Text »    PDF »
Association between Mitogen-activated Protein Kinase and the [IMAGE] Chain of the T Cell Receptor (TcR) with the SH2,3 Domain of p56[IMAGE].
A. August and B. Dupont (1996)
J. Biol. Chem. 271, 10054-10059
   Abstract »    Full Text »    PDF »
Angiotensin II Controls p21[IMAGE] Activity via pp60[IMAGE].
B. Schieffer, W. G. Paxton, Q. Chai, M. B. Marrero, and K. E. Bernstein (1996)
J. Biol. Chem. 271, 10329-10333
   Abstract »    Full Text »    PDF »
Synergistic Induction of Anchorage-independent Growth of NIH3T3 Mouse Fibroblasts by Cysteine Proteinase Inhibitors and a Tumor Promoter.
T. Hiwasa, T. Sawada, and S. Sakiyama (1996)
J. Biol. Chem. 271, 9181-9184
   Abstract »    Full Text »    PDF »


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