Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 11 June 1993:
Vol. 260. no. 5114, pp. 1649 - 1652
DOI: 10.1126/science.8503011
Prev | Table of Contents | Next

Articles

Science, Vol 260, Issue 5114, 1649-1652
Copyright © 1993 by American Association for the Advancement of Science

articles

DNA sequence determination by hybridization: a strategy for efficient large-scale sequencing

R Drmanac, S Drmanac, Z Strezoska, T Paunesku, I Labat, M Zeremski, J Snoddy, WK Funkhouser, B Koop, L Hood, and al. et

Biological and Medical Research Division, Argonne National Laboratory, IL 60439.

The concept of sequencing by hybridization (SBH) makes use of an array of all possible n-nucleotide oligomers (n-mers) to identify n-mers present in an unknown DNA sequence. Computational approaches can then be used to assemble the complete sequence. As a validation of this concept, the sequences of three DNA fragments, 343 base pairs in length, were determined with octamer oligonucleotides. Possible applications of SBH include physical mapping (ordering) of overlapping DNA clones, sequence checking, DNA fingerprinting comparisons of normal and disease-causing genes, and the identification of DNA fragments with particular sequence motifs in complementary DNA and genomic libraries. The SBH techniques may accelerate the mapping and sequencing phases of the human genome project.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Toward a universal microarray: prediction of gene expression through nearest-neighbor probe sequence identification.
T. E. Royce, J. S. Rozowsky, and M. B. Gerstein (2007)
Nucleic Acids Res.
   Abstract »    Full Text »    PDF »
Attachment of benzaldehyde-modified oligodeoxynucleotide probes to semicarbazide-coated glass.
M. A. Podyminogin, E. A. Lukhtanov, and M. W. Reed (2001)
Nucleic Acids Res. 29, 5090-5098
   Abstract »    Full Text »    PDF »
Elevated Baseline Triglyceride Levels Modulate Effects of HMGCoA Reductase Inhibitors on Plasma Lipoproteins.
S. Drmanac, D. C. Heilbron, C.R. Pullinger, M. Jafari, D. Gietzen, T. Ukrainczyk, M. H. Cho, P. H. Frost, K. Siradze, R. T. Drmanac, et al. (2001)
Journal of Cardiovascular Pharmacology and Therapeutics 6, 47-56
   Abstract »    PDF »
Molecular biological methods in diagnosis and treatment of liver diseases.
H. J. Worman (1997)
Clin. Chem. 43, 1476-1486
   Abstract »    Full Text »    PDF »
Detecting CFTR gene mutations by using primer oligo base extension and mass spectrometry.
A. Braun, D. P. Little, and H. Koster (1997)
Clin. Chem. 43, 1151-1158
   Abstract »    Full Text »    PDF »
Organization and Regulatory Aspects of the Human Intestinal Mucin Gene (MUC2) Locus.
A. Velcich, L. Palumbo, L. Selleri, G. Evans, and L. Augenlicht (1997)
J. Biol. Chem. 272, 7968-7976
   Abstract »    Full Text »    PDF »
A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization..
D Shalon, S J Smith, and P O Brown (1996)
Genome Res. 6, 639-645
   Abstract »    PDF »
Optical Melting of 128 Octamer DNA Duplexes.
M. J. Doktycz, M. D. Morris, S. J. Dormady, K. L. Beattie, and K. B. Jacobson (1995)
J. Biol. Chem. 270, 8439-8445
   Abstract »    Full Text »    PDF »
The future of DNA sequencing.
L. Smith (1993)
Science 262, 530-532
   PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)