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Science 23 April 1993:
Vol. 260. no. 5107, pp. 533 - 536
DOI: 10.1126/science.8475384
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Articles

Science, Vol 260, Issue 5107, 533-536
Copyright © 1993 by American Association for the Advancement of Science

articles

Knotting of a DNA chain during ring closure

SY Shaw and JC Wang

Harvard University, Department of Biochemistry and Molecular Biology, Cambridge, MA 02138.

The formation of knotted species on random ring closure of two DNAs that are 5.6 kilobase pairs (kbp) and 8.6 kbp in length was measured, and these data were used to calculate the effective DNA helix diameter as a function of sodium ion and magnesium ion concentration. In the presence of more than 50 mM magnesium ion, interactions between DNA segments appear to be attractive rather than repulsive. The free energy of formation of relaxed trefoil and figure-eight DNA knots and of supercoiled trefoil DNA knots was also evaluated.


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