Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 23 April 1993:
Vol. 260. no. 5107, pp. 530 - 533
DOI: 10.1126/science.8475383
Prev | Table of Contents | Next

Articles

Science, Vol 260, Issue 5107, 530-533
Copyright © 1993 by American Association for the Advancement of Science

articles

Molecular basis for specific recognition of both RNA and DNA by a zinc finger protein

KR Clemens, V Wolf, SJ McBryant, P Zhang, X Liao, PE Wright, and JM Gottesfeld

Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.

Transcription factor IIIA (TFIIIA) from Xenopus oocytes binds both the internal control region of the 5S ribosomal RNA genes and the 5S RNA transcript itself. The nucleic acid binding domain of TFIIIA contains nine tandemly repeated zinc finger motifs. A series of precisely truncated forms of this protein have been constructed and assayed for 5S RNA and DNA binding. Different sets of zinc fingers were found to be responsible for high affinity interactions with RNA and with DNA. These results explain how a single protein can exhibit equal affinities for these two very different nucleic acids.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Dicistronic tRNA-5S rRNA genes in Yarrowia lipolytica: an alternative TFIIIA-independent way for expression of 5S rRNA genes.
J. Acker, C. Ozanne, R. Kachouri-Lafond, C. Gaillardin, C. Neuveglise, and C. Marck (2008)
Nucleic Acids Res. 36, 5832-5844
   Abstract »    Full Text »    PDF »
The Evf-2 noncoding RNA is transcribed from the Dlx-5/6 ultraconserved region and functions as a Dlx-2 transcriptional coactivator.
J. Feng, C. Bi, B. S. Clark, R. Mady, P. Shah, and J. D. Kohtz (2006)
Genes & Dev. 20, 1470-1484
   Abstract »    Full Text »    PDF »
APBP-1, a DNA/RNA-binding Protein, Interacts with the Chick Aggrecan Regulatory Region.
E. W. Pirok III, M. S. Domowicz, J. Henry, Y. Wang, M. Santore, M. M. Mueller, and N. B. Schwartz (2005)
J. Biol. Chem. 280, 35606-35616
   Abstract »    Full Text »    PDF »
Is There a Dynamic DNA-Protein Interface in the Transcription Factor IIIA-5 S rRNA Gene Complex?.
K. L. Brady and D. R. Setzer (2005)
J. Biol. Chem. 280, 16115-16124
   Abstract »    Full Text »    PDF »
RoXaN, a Novel Cellular Protein Containing TPR, LD, and Zinc Finger Motifs, Forms a Ternary Complex with Eukaryotic Initiation Factor 4G and Rotavirus NSP3.
D. Vitour, P. Lindenbaum, P. Vende, M. M. Becker, and D. Poncet (2004)
J. Virol. 78, 3851-3862
   Abstract »    Full Text »    PDF »
Transcriptional activation of endoglin and transforming growth factor-beta signaling components by cooperative interaction between Sp1 and KLF6: their potential role in the response to vascular injury.
L. M. Botella, T. Sanchez-Elsner, F. Sanz-Rodriguez, S. Kojima, J. Shimada, M. Guerrero-Esteo, M. P. Cooreman, V. Ratziu, C. Langa, C. P. H. Vary, et al. (2002)
Blood 100, 4001-4010
   Abstract »    Full Text »    PDF »
Having it both ways: transcription factors that bind DNA and RNA.
L. A. Cassiday and L. J. Maher III (2002)
Nucleic Acids Res. 30, 4118-4126
   Abstract »    Full Text »    PDF »
The Bacterial Histone-like Protein HU Specifically Recognizes Similar Structures in All Nucleic Acids. DNA, RNA, AND THEIR HYBRIDS.
A. Balandina, D. Kamashev, and J. Rouviere-Yaniv (2002)
J. Biol. Chem. 277, 27622-27628
   Abstract »    Full Text »    PDF »
Identification and characterization of transcription factor IIIA from Schizosaccharomyces pombe.
D. B. Schulman and D. R. Setzer (2002)
Nucleic Acids Res. 30, 2772-2781
   Abstract »    Full Text »    PDF »
Molecular basis for modulation of biological function by alternate splicing of the Wilms' tumor suppressor protein.
J. H. Laity, H. J. Dyson, and P. E. Wright (2000)
PNAS 97, 11932-11935
   Abstract »    Full Text »    PDF »
Identification of a transcription factor IIIA-interacting protein.
R. J. Moreland, M. E. Dresser, J. S. Rodgers, B. A. Roe, J. W. Conaway, R. C. Conaway, and J. S. Hanas (2000)
Nucleic Acids Res. 28, 1986-1993
   Abstract »    Full Text »    PDF »
Structure-based design of an RNA-binding zinc finger.
D. J. McColl, C. D. Honchell, and A. D. Frankel (1999)
PNAS 96, 9521-9526
   Abstract »    Full Text »    PDF »
Identification of the RNA-Binding, Dimerization, and eIF4GI-Binding Domains of Rotavirus Nonstructural Protein NSP3.
M. Piron, T. Delaunay, J. Grosclaude, and D. Poncet (1999)
J. Virol. 73, 5411-5421
   Abstract »    Full Text »    PDF »
A20 Inhibits NF-kappa B Activation in Endothelial Cells Without Sensitizing to Tumor Necrosis Factor-Mediated Apoptosis.
C. Ferran, D. M. Stroka, A. Z. Badrichani, J. T. Cooper, C. J. Wrighton, M. Soares, S. T. Grey, and F. H. Bach (1998)
Blood 91, 2249-2258
   Abstract »    Full Text »    PDF »
Getting a handhold on DNA: Design of poly-zinc finger proteins with femtomolar dissociation constants.
J.-S. Kim and C. O. Pabo (1998)
PNAS 95, 2812-2817
   Abstract »    Full Text »    PDF »
A Hydrophobic Segment within the 81-Amino-Acid Domain of TFIIIA from Saccharomyces cerevisiae Is Essential for Its Transcription Factor Activity.
O. Rowland and J. Segall (1998)
Mol. Cell. Biol. 18, 420-432
   Abstract »    Full Text »    PDF »
Energetically Unfavorable Interactions among the Zinc Fingers of Transcription Factor IIIA When Bound to the 5 S rRNA Gene.
D. G. Kehres, G. S. Subramanyan, V. S. Hung, G. W. Rogers Jr., and D. R. Setzer (1997)
J. Biol. Chem. 272, 20152-20161
   Abstract »    Full Text »    PDF »
Phage Display of RNA Binding Zinc Fingers from Transcription FactorIIIA.
W. J. Friesen and M. K. Darby (1997)
J. Biol. Chem. 272, 10994-10997
   Abstract »    Full Text »    PDF »
A zinc finger directory for high-affinity DNA recognition.
A. C. Jamieson, H. Wang, and S.-H. Kim (1996)
PNAS 93, 12834-12839
   Abstract »    Full Text »    PDF »
Transcription factor IIIA (TFIIIA) in the second decade.
B. S. Shastry (1996)
J. Cell Sci. 109, 535-539
   Abstract »    PDF »
Analysis of the Binding of Xenopus Transcription Factor IIIA to Oocyte 5 S rRNA and to the 5 S rRNA Gene.
S. L. Rawlings, G. D. Matt, and P. W. Huber (1996)
J. Biol. Chem. 271, 869-877
   Abstract »    Full Text »    PDF »
The K Protein Domain That Recruits the Interleukin 1-responsive K Protein Kinase Lies Adjacent to a Cluster of c-Src and Vav SH3-binding Sites.
I. Van Seuningen, J. Ostrowski, X. R. Bustelo, P. R. Sleath, and K. Bomsztyk (1995)
J. Biol. Chem. 270, 26976-26985
   Abstract »    Full Text »    PDF »
Analysis of the Binding of Xenopus Ribosomal Protein L5 to Oocyte 5 S rRNA.
J. B. Scripture and P. W. Huber (1995)
J. Biol. Chem. 270, 27358-27365
   Abstract »    Full Text »    PDF »
The Interaction of TFIIIA with Specific RNA-DNA Heteroduplexes.
K. P. Nightingale and A. P. Wolffe (1995)
J. Biol. Chem. 270, 22665-22668
   Abstract »    Full Text »    PDF »
Specific DNA-RNA hybrid binding by zinc finger proteins.
Y Shi and J. Berg (1995)
Science 268, 282-284
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)