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Science 9 April 1993:
Vol. 260. no. 5105, pp. 214 - 216
DOI: 10.1126/science.8097061
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Articles

Science, Vol 260, Issue 5105, 214-216
Copyright © 1993 by American Association for the Advancement of Science

articles

Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew

A Stierle, G Strobel, and D Stierle

Department of Plant Pathology, Montana State University, Bozeman 59717.

Taxomyces andreanae, a fungal endophyte, was isolated from the phloem (inner bark) of the Pacific yew, Taxus brevifolia. The fungus is hyphomyceteous and, when grown in a semi-synthetic liquid medium, produced taxol and related compounds. Taxol was identified by mass spectrometry, chromatography, and reactivity with monoclonal antibodies specific for taxol. Both [1-14C]acetic acid and L-[U-14C]phenylalanine served as precursors of [14C]taxol in fungal cultures. No taxol was detected in zero-time cultures or in the small agar plugs used to inoculate the culture flasks.


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