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Science 26 February 1993:
Vol. 259. no. 5099, pp. 1314 - 1318
DOI: 10.1126/science.7680491
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Articles

Science, Vol 259, Issue 5099, 1314-1318
Copyright © 1993 by American Association for the Advancement of Science

articles

Inhibition of Rev-mediated HIV-1 expression by an RNA binding protein encoded by the interferon-inducible 9-27 gene

P Constantoulakis, M Campbell, BK Felber, G Nasioulas, E Afonina, and GN Pavlakis

Human Retrovirus Section, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702.

Interferon inhibits expression of human immunodeficiency virus type-1 (HIV-1) through unknown mechanisms. A gene inducible by interferon-alpha (IFN-alpha) and interferon-gamma (IFN-gamma) was isolated by screening of a human complementary DNA library for proteins binding to the Rev-responsive element (RRE) of HIV-1. The product of this gene, RBP9-27, was shown to bind RNA in vitro and to inhibit HIV-1 expression after transfection into human cells. RBP9-27 primarily inhibited Rev-dependent posttranscriptional steps of viral gene expression. Thus, RBP9-27 is a cellular factor that antagonizes Rev function. These results suggest an interferon-induced antiviral mechanism operating through the induction of RNA binding proteins such as RBP9-27. Elucidation of RBP9-27 function may lead to a better understanding of the mechanism of interferon action during HIV-1 infection.


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