Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 19 February 1993:
Vol. 259. no. 5098, pp. 1173 - 1175
DOI: 10.1126/science.8382377
Prev | Table of Contents | Next

Articles

Science, Vol 259, Issue 5098, 1173-1175
Copyright © 1993 by American Association for the Advancement of Science

articles

Phosphorylation and modulation of a kainate receptor (GluR6) by cAMP-dependent protein kinase

LY Wang, FA Taverna, XP Huang, JF MacDonald, and DR Hampson

Department of Physiology, University of Toronto, Ontario, Canada.

Ligand-gated ion channels gated by glutamate constitute the major excitatory neurotransmitter system in the mammalian brain. The functional modulation of GluR6, a kainate-activated glutamate receptor, by adenosine 3',5'-monophosphate-dependent protein kinase A (PKA) was examined with receptors expressed in human embryonic kidney cells. Kainate-evoked currents underwent a rapid desensitization that was blocked by lectins. Kainate currents were potentiated by intracellular perfusion of PKA, and this potentiation was blocked by co-application of an inhibitory peptide. Site-directed mutagenesis was used to identify the site or sites of phosphorylation on GluR6. Although mutagenesis of two serine residues, Ser684 and Ser666, was required for complete abolition of the PKA-induced potentiation, Ser684 may be the preferred site of phosphorylation in native GluR6 receptor complexes. These results indicate that glutamate receptor function can be directly modulated by protein phosphorylation and suggest that a dynamic regulation of excitatory receptors could be associated with some forms of learning and memory in the mammalian brain.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Platelet-derived Growth Factor Selectively Inhibits NR2B-containing N-Methyl-D-aspartate Receptors in CA1 Hippocampal Neurons.
M. A. Beazely, A. Lim, H. Li, C. Trepanier, X. Chen, B. Sidhu, and J. F. MacDonald (2009)
J. Biol. Chem. 284, 8054-8063
   Abstract »    Full Text »    PDF »
Activation of Pedunculopontine Tegmental PKA Prevents GABAB Receptor Activation-Mediated Rapid Eye Movement Sleep Suppression in the Freely Moving Rat.
S. Datta (2007)
J Neurophysiol 97, 3841-3850
   Abstract »    Full Text »    PDF »
Activation of pedunculopontine tegmental protein kinase a: a mechanism for rapid eye movement sleep generation in the freely moving rat..
R. S. Bandyopadhya, S. Datta, and S. Saha (2006)
J. Neurosci. 26, 8931-8942
   Abstract »    Full Text »    PDF »
Postsynaptic Mechanisms Are Essential for Forskolin-Induced Potentiation of Synaptic Transmission.
I. V. Sokolova, H. A. Lester, and N. Davidson (2006)
J Neurophysiol 95, 2570-2579
   Abstract »    Full Text »    PDF »
Novel Role of Brain Stem Pedunculopontine Tegmental Adenylyl Cyclase in the Regulation of Spontaneous REM Sleep in the Freely Moving Rat.
S. Datta and S. L. Prutzman (2005)
J Neurophysiol 94, 1928-1937
   Abstract »    Full Text »    PDF »
Glucocorticoid adrenal steroids and glucocorticoid-inducible kinase isoforms in the regulation of GluR6 expression.
N. Strutz-Seebohm, G. Seebohm, E. Shumilina, A. F. Mack, H.-J. Wagner, A. Lampert, F. Grahammer, G. Henke, L. Just, T. Skutella, et al. (2005)
J. Physiol. 565, 391-401
   Abstract »    Full Text »    PDF »
Presynaptic kainate receptor facilitation of glutamate release involves protein kinase A in the rat hippocampus.
A. Rodriguez-Moreno and T. S. Sihra (2004)
J. Physiol. 557, 733-745
   Abstract »    Full Text »    PDF »
Regulation of kainate receptors by protein kinase C and metabotropic glutamate receptors.
K. Cho, J. C Francis, H. Hirbec, K. Dev, M. W Brown, J. M Henley, and Z. I Bashir (2003)
J. Physiol. 548, 723-730
   Abstract »    Full Text »    PDF »
Modulation of AMPA receptors by cAMP-dependent protein kinase in PreBotzinger complex inspiratory neurons regulates respiratory rhythm in the rat.
X. M Shao, Q. Ge, and J. L Feldman (2003)
J. Physiol. 547, 543-553
   Abstract »    Full Text »    PDF »
AMPA Receptors and Kainate Receptors Encode Different Features of Afferent Activity.
M. Frerking and P. Ohliger-Frerking (2002)
J. Neurosci. 22, 7434-7443
   Abstract »    Full Text »    PDF »
Molecular Physiology of Kainate Receptors.
J. Lerma, A. V. Paternain, A. Rodriguez-Moreno, and J. C. Lopez-Garcia (2001)
Physiol Rev 81, 971-998
   Abstract »    Full Text »    PDF »
NMDA-Dependent Modulation of Hippocampal Kainate Receptors by Calcineurin and Ca2+/Calmodulin-Dependent Protein Kinase.
A. Ghetti and S. F. Heinemann (2000)
J. Neurosci. 20, 2766-2773
   Abstract »    Full Text »    PDF »
Cysteine Mutagenesis and Homology Modeling of the Ligand-binding Site of a Kainate-binding Protein.
Z. G. Wo, K. K. Chohan, H. Chen, M. J. Sutcliffe, and R. E. Oswald (1999)
J. Biol. Chem. 274, 37210-37218
   Abstract »    Full Text »    PDF »
Neuroprotection at Drosophila Synapses Conferred by Prior Heat Shock.
S. Karunanithi, J. W. Barclay, R. M. Robertson, I. R. Brown, and H. L. Atwood (1999)
J. Neurosci. 19, 4360-4369
   Abstract »    Full Text »    PDF »
Ca2+/calmodulin-kinase II enhances channel conductance of alpha -amino-3-hydroxy-5-methyl-4-isoxazolepropionate type glutamate receptors.
V. Derkach, A. Barria, and T. R. Soderling (1999)
PNAS 96, 3269-3274
   Abstract »    Full Text »    PDF »
Metabotropic Glutamate Receptor Modulation of Glutamate Responses in the Suprachiasmatic Nucleus.
L. L. Haak (1999)
J Neurophysiol 81, 1308-1317
   Abstract »    Full Text »    PDF »
The Glutamate Receptor Ion Channels.
R. Dingledine, K. Borges, D. Bowie, and S. F. Traynelis (1999)
Pharmacol. Rev. 51, 7-62
   Abstract »    Full Text »    PDF »
Plasticity of First-Order Sensory Synapses: Interactions between Homosynaptic Long-Term Potentiation and Heterosynaptically Evoked Dopaminergic Potentiation.
S. S. Kumar and D. S. Faber (1999)
J. Neurosci. 19, 1620-1635
   Abstract »    Full Text »    PDF »
Agonist-Induced Changes in Substituted Cysteine Accessibility Reveal Dynamic Extracellular Structure of M3-M4 Loop of Glutamate Receptor GluR6.
S. S. Basiry, P. Mendoza, P. D. Lee, and L. A. Raymond (1999)
J. Neurosci. 19, 644-652
   Abstract »    Full Text »    PDF »
Involvement of simultaneous multiple transcription factor expression, including cAMP responsive element binding protein and OCT-1, for synovial cell outgrowth in patients with rheumatoid arthritis.
S. Wakisaka, N. Suzuki, M. Takeno, Y. Takeba, H. Nagafuchi, N. Saito, H. Hashimoto, T. Tomita, T. Ochi, and T. Sakane (1998)
Ann Rheum Dis 57, 487-494
   Abstract »    Full Text »
An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals.
T. Genoud, A. J. Millar, N. Nishizawa, S. A. Kay, E. Schäfer, A. Nagatani, and N.-H. Chua (1998)
PLANT CELL 10, 889-904
   Abstract »    Full Text »
Identification of the Ca2+/Calmodulin-dependent Protein Kinase II Regulatory Phosphorylation Site in the alpha -Amino-3-hydroxyl-5-methyl4-isoxazole-propionate-type Glutamate Receptor.
A. Barria, V. Derkach, and T. Soderling (1997)
J. Biol. Chem. 272, 32727-32730
   Abstract »    Full Text »    PDF »
NMDA-Independent LTP by Adenosine A2 Receptor-Mediated Postsynaptic AMPA Potentiation in Hippocampus.
K. Kessey and D. J. Mogul (1997)
J Neurophysiol 78, 1965-1972
   Abstract »    Full Text »    PDF »
Dopaminergic Modulation of Excitatory Postsynaptic Currents in Rat Neostriatal Neurons.
M. Umemiya and L. A. Raymond (1997)
J Neurophysiol 78, 1248-1255
   Abstract »    Full Text »    PDF »
Involvement of cAMP-Dependent Protein Kinase in µ-Opioid Modulation of NMDA-Mediated Synaptic Currents.
C.-W. Xie and D. V. Lewis (1997)
J Neurophysiol 78, 759-766
   Abstract »    Full Text »    PDF »
An Alanine Residue in the M3-M4 Linker Lines the Glycine Binding Pocket of the N-Methyl-D-aspartate Receptor.
M. W. Wood, H. M.A. VanDongen, and A. M.J. VanDongen (1997)
J. Biol. Chem. 272, 3532-3537
   Abstract »    Full Text »    PDF »
Persistent phosphorylation parallels long-term desensitization of cerebellar purkinje cell AMPA-type glutamate receptors..
K Nakazawa, S Mikawa, and M Ito (1997)
Learn. Mem. 3, 578-591
   Abstract »    PDF »
Transient Activation of Cyclic AMP-dependent Protein Kinase during Hippocampal Long-term Potentiation.
E. D. Roberson and J. D. Sweatt (1996)
J. Biol. Chem. 271, 30436-30441
   Abstract »    Full Text »    PDF »
Interaction of the N-Methyl--aspartate Receptor Complex with a Novel Synapse-associated Protein, SAP102.
L.-F. Lau, A. Mammen, M. D. Ehlers, S. Kindler, W. J. Chung, C. C. Garner, and R. L. Huganir (1996)
J. Biol. Chem. 271, 21622-21628
   Abstract »    Full Text »    PDF »
Characterization of the Ligand-binding Domains of Glutamate Receptor (GluR)-B and GluR-D Subunits Expressed in Escherichia coli as Periplasmic Proteins.
M. Arvola and K. Keinanen (1996)
J. Biol. Chem. 271, 15527-15532
   Abstract »    Full Text »    PDF »
17beta -Estradiol Potentiates Kainate-Induced Currents via Activation of the cAMP Cascade.
Q. Gu and R. L. Moss (1996)
J. Neurosci. 16, 3620-3629
   Abstract »    Full Text »    PDF »
Promoter Sequences in the RIbeta Subunit Gene of cAMP-dependent Protein Kinase Required for Transgene Expression in Mouse Brain.
C. H. Clegg, H. S. Haugen, and L. F. Boring (1996)
J. Biol. Chem. 271, 1638-1644
   Abstract »    Full Text »    PDF »
Effects of a conditional Drosophila PKA mutant on olfactory learning and memory..
W Li, T Tully, and D Kalderon (1996)
Learn. Mem. 2, 320-333
   Abstract »    PDF »
The Responsiveness of a Tetracycline-sensitive Expression System Differs in Different Cell Lines.
J. R. Howe, B. V. Skryabin, S. M. Belcher, C. A. Zerillo, and C. Schmauss (1995)
J. Biol. Chem. 270, 14168-14174
   Abstract »    Full Text »    PDF »
A Topological Analysis of Goldfish Kainate Receptors Predicts Three Transmembrane Segments.
Z. G. Wo and R. E. Oswald (1995)
J. Biol. Chem. 270, 2000-2009
   Abstract »    Full Text »    PDF »
Mediation of hippocampal mossy fiber long-term potentiation by cyclic AMP.
M. Weisskopf, P. Castillo, R. Zalutsky, and R. Nicoll (1994)
Science 265, 1878-1882
   Abstract »    PDF »
An essential role for protein phosphatases in hippocampal long-term depression.
R. Mulkey, C. Herron, and R. Malenka (1993)
Science 261, 1051-1055
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)