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Science 12 February 1993:
Vol. 259. no. 5097, pp. 988 - 990
DOI: 10.1126/science.8382374
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Articles

Science, Vol 259, Issue 5097, 988-990
Copyright © 1993 by American Association for the Advancement of Science

articles

An adenovirus vector for gene transfer into neurons and glia in the brain

G Le Gal La Salle, JJ Robert, S Berrard, V Ridoux, LD Stratford-Perricaudet, M Perricaudet, and J Mallet

Institut Alfred Fessard, Unite Propre de Recherche 2212, Centre National de la Recherche Scientifique (CNRS), Gif sur Yvette, France.

The efficient introduction of genetic material into quiescent nerve cells is important in the study of brain function and for gene therapy of neurological disorders. A replication-deficient adenoviral vector that contained a reporter gene encoding beta-galactosidase infected rat nerve cells in vitro and in vivo. beta-Galactosidase was expressed in almost all sympathetic neurons and astrocytes in culture. After stereotactic inoculations into the rat hippocampus and the substantia nigra, beta-galactosidase activity was detected for 2 months. Infected cells were identified as microglial cells, astrocytes, or neurons with anatomical, morphological, and immunohistochemical criteria. No obvious cytopathic effect was observed.


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