Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 5 February 1993:
Vol. 259. no. 5096, pp. 829 - 832
DOI: 10.1126/science.8430337
Prev | Table of Contents | Next

Articles

Science, Vol 259, Issue 5096, 829-832
Copyright © 1993 by American Association for the Advancement of Science

articles

Role of the acylated amino terminus of recoverin in Ca(2+)-dependent membrane interaction

AM Dizhoor, CK Chen, E Olshevskaya, VV Sinelnikova, P Phillipov, and JB Hurley

Department of Biochemistry, University of Washington, Seattle 98195.

Recoverin, a calcium ion (Ca2+)-binding protein of vertebrate photoreceptors, binds to photoreceptor membranes when the Ca2+ concentration is greater than 1 micromolar. This interaction requires a fatty acyl residue covalently linked to the recoverin amino (NH2)-terminus. Removal of the acyl residue, either by proteolytic cleavage of the NH2-terminus or by production of nonacylated recoverin, prevented recoverin from binding to membranes. The acylated recoverin NH2-terminus could be cleaved by trypsin only when Ca2+ was bound to recoverin. These results suggest that the hydrophobic NH2-terminus is constrained in Ca(2+)-free recoverin and liberated by Ca2+ binding. The hydrophobic acyl moiety of recoverin may interact with the membrane only when recoverin binds Ca2+.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Structural Insights into Membrane Targeting by the Flagellar Calcium-binding Protein (FCaBP), a Myristoylated and Palmitoylated Calcium Sensor in Trypanosoma cruzi.
J. N. Wingard, J. Ladner, M. Vanarotti, A. J. Fisher, H. Robinson, K. T. Buchanan, D. M. Engman, and J. B. Ames (2008)
J. Biol. Chem. 283, 23388-23396
   Abstract »    Full Text »    PDF »
Structural Insights into Activation of Phosphatidylinositol 4-Kinase (Pik1) by Yeast Frequenin (Frq1).
T. Strahl, I. G. Huttner, J. D. Lusin, M. Osawa, D. King, J. Thorner, and J. B. Ames (2007)
J. Biol. Chem. 282, 30949-30959
   Abstract »    Full Text »    PDF »
Structural Basis for Calcium-induced Inhibition of Rhodopsin Kinase by Recoverin.
J. B. Ames, K. Levay, J. N. Wingard, J. D. Lusin, and V. Z. Slepak (2006)
J. Biol. Chem. 281, 37237-37245
   Abstract »    Full Text »    PDF »
Recoverin Binds Exclusively to an Amphipathic Peptide at the N Terminus of Rhodopsin Kinase, Inhibiting Rhodopsin Phosphorylation without Affecting Catalytic Activity of the Kinase.
M. K. Higgins, D. D. Oprian, and G. F. X. Schertler (2006)
J. Biol. Chem. 281, 19426-19432
   Abstract »    Full Text »    PDF »
A Flagellum-specific Calcium Sensor.
K. T. Buchanan, J. B. Ames, S. H. Asfaw, J. N. Wingard, C. L. Olson, P. T. Campana, A. P. U. Araujo, and D. M. Engman (2005)
J. Biol. Chem. 280, 40104-40111
   Abstract »    Full Text »    PDF »
Differential Regulation of CaV2.1 Channels by Calcium-Binding Protein 1 and Visinin-Like Protein-2 Requires N-Terminal Myristoylation.
A. P. Few, N. J. Lautermilch, R. E. Westenbroek, T. Scheuer, and W. A. Catterall (2005)
J. Neurosci. 25, 7071-7080
   Abstract »    Full Text »    PDF »
Ca2+-dependent Conformational Changes in Guanylyl Cyclase-activating Protein 2 (GCAP-2) Revealed by Site-specific Phosphorylation and Partial Proteolysis.
I. V. Peshenko, E. V. Olshevskaya, and A. M. Dizhoor (2004)
J. Biol. Chem. 279, 50342-50349
   Abstract »    Full Text »    PDF »
Expression, Localization, and Correlation of N-Myristoyltransferase and Its Inhibitor in Bovine Eye.
A. Shrivastav, M. K. Pasha, P. Selvakumar, B. Singh, and R. K. Sharma (2004)
Invest. Ophthalmol. Vis. Sci. 45, 1674-1679
   Abstract »    Full Text »    PDF »
Fission Yeast Homolog of Neuronal Calcium Sensor-1 (Ncs1p) Regulates Sporulation and Confers Calcium Tolerance.
N. Hamasaki-Katagiri, T. Molchanova, K. Takeda, and J. B. Ames (2004)
J. Biol. Chem. 279, 12744-12754
   Abstract »    Full Text »    PDF »
Production of N-Lauroylated G Protein {alpha}-Subunit in Sf9 Insect Cells: The Type of N-Acyl Group of G{alpha} Influences G Protein-Mediated Signal Transduction.
Y. Hashimoto, T. Matsuda, Y. Matsuura, T. Haga, and Y. Fukada (2004)
J. Biochem. 135, 319-329
   Abstract »    Full Text »    PDF »
Impact of N-terminal Myristoylation on the Ca2+-dependent Conformational Transition in Recoverin.
O. H. Weiergraber, I. I. Senin, P. P. Philippov, J. Granzin, and K.-W. Koch (2003)
J. Biol. Chem. 278, 22972-22979
   Abstract »    Full Text »    PDF »
Ca2+-Myristoyl Switch in the Neuronal Calcium Sensor Recoverin Requires Different Functions of Ca2+-binding Sites.
I. I. Senin, T. Fischer, K. E. Komolov, D. V. Zinchenko, P. P. Philippov, and K.-W. Koch (2002)
J. Biol. Chem. 277, 50365-50372
   Abstract »    Full Text »    PDF »
SOS3 Function in Plant Salt Tolerance Requires N-Myristoylation and Calcium Binding.
M. Ishitani, J. Liu, U. Halfter, C.-S. Kim, W. Shi, and J.-K. Zhu (2000)
PLANT CELL 12, 1667-1678
   Abstract »    Full Text »
Mobilization of the A-kinase N-myristate through an isoform-specific intermolecular switch.
M. Gangal, T. Clifford, J. Deich, X. Cheng, S. S. Taylor, and D. A. Johnson (1999)
PNAS 96, 12394-12399
   Abstract »    Full Text »    PDF »
Three-dimensional Structure of Guanylyl Cyclase Activating Protein-2, a Calcium-sensitive Modulator of Photoreceptor Guanylyl Cyclases.
J. B. Ames, A. M. Dizhoor, M. Ikura, K. Palczewski, and L. Stryer (1999)
J. Biol. Chem. 274, 19329-19337
   Abstract »    Full Text »    PDF »
Opposing Effects of Human Immunodeficiency Virus Type 1 Matrix Mutations Support a Myristyl Switch Model of Gag Membrane Targeting.
J.-C. Paillart and H. G. Gottlinger (1999)
J. Virol. 73, 2604-2612
   Abstract »    Full Text »    PDF »
Identification of a Gialpha Binding Site on Type V Adenylyl Cyclase.
C. W. Dessauer, J. J. G. Tesmer, S. R. Sprang, and A. G. Gilman (1998)
J. Biol. Chem. 273, 25831-25839
   Abstract »    Full Text »    PDF »
The Role of Calcium-binding Sites in S-modulin Function.
S. Matsuda, O. Hisatomi, T. Ishino, Y. Kobayashi, and F. Tokunaga (1998)
J. Biol. Chem. 273, 20223-20227
   Abstract »    Full Text »    PDF »
The effect of recombinant recoverin on the photoresponse of truncated rod photoreceptors.
M. A. Erickson, L. Lagnado, S. Zozulya, T. A. Neubert, L. Stryer, and D. A. Baylor (1998)
PNAS 95, 6474-6479
   Abstract »    Full Text »    PDF »
Onset of Feedback Reactions Underlying Vertebrate Rod Photoreceptor Light Adaptation.
P. D. Calvert, T. W. Ho, Y. M. LeFebvre, and V. Y. Arshavsky (1998)
J. Gen. Physiol. 111, 39-51
   Abstract »    Full Text »    PDF »
Posttranslational Modifications of the 5'-AMP-activated Protein Kinase beta 1 Subunit.
K. I. Mitchelhill, B. J. Michell, C. M. House, D. Stapleton, J. Dyck, J. Gamble, C. Ullrich, L. A. Witters, and B. E. Kemp (1997)
J. Biol. Chem. 272, 24475-24479
   Abstract »    Full Text »    PDF »
Calcium Binding, but Not a Calcium-Myristoyl Switch, Controls the Ability of Guanylyl Cyclase-activating Protein GCAP-2 to Regulate Photoreceptor Guanylyl Cyclase.
E. V. Olshevskaya, R. E. Hughes, J. B. Hurley, and A. M. Dizhoor (1997)
J. Biol. Chem. 272, 14327-14333
   Abstract »    Full Text »    PDF »
Inactivation of EF-hands Makes GCAP-2 (p24) a Constitutive Activator of Photoreceptor Guanylyl Cyclase by Preventing a Ca2+-induced ``Activator-to-Inhibitor'' Transition.
A. M. Dizhoor and J. B. Hurley (1996)
J. Biol. Chem. 271, 19346-19350
   Abstract »    Full Text »    PDF »
Molecular Determinants of the Myristoyl-electrostatic Switch of MARCKS.
J. T. Seykora, M. M. Myat, L.-A. H. Allen, J. V. Ravetch, and A. Aderem (1996)
J. Biol. Chem. 271, 18797-18802
   Abstract »    Full Text »    PDF »
A Novel Ca[IMAGE]-binding Protein, p22, Is Required for Constitutive Membrane Traffic.
M. R. Barroso, K. K. Bernd, N. D. DeWitt, A. Chang, K. Mills, and E. S. Sztul (1996)
J. Biol. Chem. 271, 10183-10187
   Abstract »    Full Text »    PDF »
Drosophila Neurocalcin, a Fatty Acylated, Ca[IMAGE]-binding Protein that Associates with Membranes and Inhibits in Vitro Phosphorylation of Bovine Rhodopsin.
E. Faurobert, C.-K. Chen, J. B. Hurley, and D. H.-F. Teng (1996)
J. Biol. Chem. 271, 10256-10262
   Abstract »    Full Text »    PDF »
Myristoylation of Calcineurin B Is Not Required for Function or Interaction with Immunophilin-Immunosuppressant Complexes in the Yeast Saccharomyces cerevisiae.
D. Zhu, M. E. Cardenas, and J. Heitman (1995)
J. Biol. Chem. 270, 24831-24838
   Abstract »    Full Text »    PDF »
Cloning, Sequencing, and Expression of a 24-kDa Ca[IMAGE]-binding Protein Activating Photoreceptor Guanylyl Cyclase.
A. M. Dizhoor, E. V. Olshevskaya, W. J. Henzel, S. C. Wong, J. T. Stults, I. Ankoudinova, and J. B. Hurley (1995)
J. Biol. Chem. 270, 25200-25206
   Abstract »    Full Text »    PDF »
Rhodopsin Kinase Inhibition by Recoverin.
P. D. Calvert, V. A. Klenchin, and M. D. Bownds (1995)
J. Biol. Chem. 270, 24127-24129
   Abstract »    Full Text »    PDF »
Ca[IMAGE]-dependent Interaction of Recoverin with Rhodopsin Kinase.
C.-K. Chen, J. Inglese, R. J. Lefkowitz, and J. B. Hurley (1995)
J. Biol. Chem. 270, 18060-18066
   Abstract »    Full Text »    PDF »
Inhibition of Rhodopsin Kinase by Recoverin.
V. A. Klenchin, P. D. Calvert, and M. D. Bownds (1995)
J. Biol. Chem. 270, 16147-16152
   Abstract »    Full Text »    PDF »
Role of Heterogeneous N-terminal Acylation of Recoverin in Rhodopsin Phosphorylation.
K. Sanada, K. Kokame, Tôr. Yoshizawa, T. Takao, Y. Shimonishi, and Y. Fukada (1995)
J. Biol. Chem. 270, 15459-15462
   Abstract »    Full Text »    PDF »
The Myristoylated Amino Terminus of ADP-ribosylation Factor 1 Is a Phospholipid- and GTP-sensitive Switch.
P. A. Randazzo, T. Terui, S. Sturch, H. M. Fales, A. G. Ferrige, and R. A. Kahn (1995)
J. Biol. Chem. 270, 14809-14815
   Abstract »    Full Text »    PDF »
Protein lipidation in cell signaling.
P. Casey (1995)
Science 268, 221-225
   Abstract »    PDF »
Amino-terminal Myristoylation Induces Cooperative Calcium Binding to Recoverin.
J. B. Ames, T. Porumb, T. Tanaka, M. Ikura, and L. Stryer (1995)
J. Biol. Chem. 270, 4526-4533
   Abstract »    Full Text »    PDF »
Calcium and Membrane Binding Properties of Bovine Neurocalcin [IMAGE] Expressed in Escherichia coli.
D. Ladant and D. Ladant (1995)
J. Biol. Chem. 270, 3179-3185
   Abstract »    Full Text »    PDF »
Cellular and Subcellular Localization, N-terminal Acylation, and Calcium Binding of Caenorhabditis elegans Protein Phosphatase with EF-hands.
P. Ramulu and J. Nathans (2001)
J. Biol. Chem. 276, 25127-25135
   Abstract »    Full Text »    PDF »
Developmental Changes in the Subcellular Localization of Calretinin.
N. J. Hack, M. C. Wride, K. M. Charters, S. B. Kater, and T. N. Parks (2000)
J. Neurosci. 20, RC67
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)