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Science 29 January 1993:
Vol. 259. no. 5095, pp. 686 - 688
DOI: 10.1126/science.8430319

Articles

Science, Vol 259, Issue 5095, 686-688
Copyright © 1993 by American Association for the Advancement of Science


articles

Selection of bacterial virulence genes that are specifically induced in host tissues

MJ Mahan, JM Slauch, and JJ Mekalanos

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

A genetic system was devised that positively selects for bacterial genes that are specifically induced when bacteria infect their host. With the pathogen Salmonella typhimurium, the genes identified by this selection show a marked induction in bacteria recovered from mouse spleen. Mutations in all ivi (in vivo-induced) genes that were tested conferred a defect in virulence. This genetic system was designed to be of general use in a wide variety of bacterial-host systems and has several applications in both vaccine and antimicrobial drug development.


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Amino Acid Transport and Metabolism in Mycobacteria: Cloning, Interruption, and Characterization of an L-Arginine/gamma -Aminobutyric Acid Permease in Mycobacterium bovis BCG.
A. Seth and N. D. Connell (2000)
J. Bacteriol. 182, 919-927
   Abstract »    Full Text »
Broad-Host-Range Shuttle Vectors for Screening of Regulated Promoter Activity in Viridans Group Streptococci: Isolation of a pH-Regulated Promoter.
A. J. M. Vriesema, R. Brinkman, J. Kok, J. Dankert, and S. A. J. Zaat (2000)
Appl. Envir. Microbiol. 66, 535-542
   Abstract »    Full Text »
Use of Lambda Phage S and R Gene Products in an Inducible Lysis System for Vibrio cholerae- and Salmonella enterica Serovar Typhimurium-Based DNA Vaccine Delivery Systems.
V. Jain and J. J. Mekalanos (2000)
Infect. Immun. 68, 986-989
   Abstract »    Full Text »    PDF »
Expression of the soxR Gene of Pseudomonas aeruginosa Is Inducible during Infection of Burn Wounds in Mice and Is Required To Cause Efficient Bacteremia.
U. Ha and S. Jin (1999)
Infect. Immun. 67, 5324-5331
   Abstract »    Full Text »    PDF »
Functional Characterization of a Serine/Threonine Protein Kinase of Pseudomonas aeruginosa.
S. T. Motley and S. Lory (1999)
Infect. Immun. 67, 5386-5394
   Abstract »    Full Text »    PDF »
Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS).
J. E. Graham and J. E. Clark-Curtiss (1999)
PNAS 96, 11554-11559
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A role for the PhoBR regulatory system homologue in the Vibrio cholerae phosphate-limitation response and intestinal colonization.
W. M. A. von Kruger, S. Humphreys, and J. M. Ketley (1999)
Microbiology 145, 2463-2475
   Abstract »    Full Text »    PDF »
Broadly Protective Vaccine for Staphylococcus aureus Based on an in Vivo-Expressed Antigen.
D. McKenney, K. L. Pouliot, Y. Wang, V. Murthy, M. Ulrich, G. Döring, J. C. Lee, D. A. Goldmann, and G. B. Pier (1999)
Science 284, 1523-1527
   Abstract »    Full Text »
Cryptococcus neoformans Differential Gene Expression Detected In Vitro and In Vivo with Green Fluorescent Protein.
M. del Poeta, D. L. Toffaletti, T. H. Rude, S. D. Sparks, J. Heitman, and J. R. Perfect (1999)
Infect. Immun. 67, 1812-1820
   Abstract »    Full Text »    PDF »



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